中文摘要：

目的：研究形觉剥夺性和光学离焦性近视豚鼠视紫红质的表达变化，探讨视紫红质表达与实验性近视眼之问的关系。方法：40只出生后1周的豚鼠随机分为形觉剥夺组和光学离焦组（n=20），形觉剥夺组单眼戴半透明（半透明薄膜贴于平镜表面）平光硬性角膜接触镜片（Rigid gass—permeable contactlens，RGP），光学离焦组单眼戴-4．0DRGP镜片，另一只眼为对照组。戴镜干预后1、2周各组分别测量屈光度、眼轴长度、玻璃体腔深度，并于上午10～12点钟取材，实时荧光定量PCR观察视紫红质mRNA的变化，Western—blot观察视紫红质的变化，进行对比比较，统计分析。结果：实验干预后1周，形觉剥夺组和光学离焦组与对照组相比各项指标无显著性差异（除形觉剥夺组屈光度以外）。实验干预后2周，与对照组相比较，形觉剥夺组和光学离焦组明显发生近视、眼轴延长、玻璃体腔加深（t：22．20、18．32、19．65、15．78、6．18、11．20，P〈0．01）；形觉剥夺组视紫红质及其mRNA表达均增加（t=17．489、14．31，P〈0．05），光学离焦组视紫红质及其mRNA表达无明显变化。结论：视紫红质的表达可能参与了形觉剥夺性近视眼的形成，而在光学离焦性近视眼中作用有限，

英文摘要：

Purpose： To investigate the rhodopsin expression in form-deprived and defocus myopia in guinea pig and study the relationship between the rhodopsin expression and experimental myopia. Methods： Fourty guinea pigs were randomized into the form-deprived group and the defocus group （n = 20）. Guinea pigs in the form-deprived group wore a diffuser（rigid gass-permeable contact lens（RGP） on one eye since one week after birth. Those in defocus group wore a -4 D RGP on one eye. The contralateral eyes were left as control. Refraction, axial length and depth of vitreous cavity were measured after 1 and 2 weeks respectively. Retina were dissected at 10- 12 o＇clock in the morning. The level of rhodopsin and its mRNA were observed through Western-blot and real- time PCR respectively. Result： There is no difference between form-deprived group, defocus group and control groups （except refraction in form-deprived group）. One week later, there is no difference between the form-deprived group, the defocus group and the control groups （except refraction in form-deprived group）. Two weeks later, eyes in the form-deprived group and the defocus group became myopic. Its axial length lengthened and depth of vitreous cavity appeared deep. The form-deprived groups showed an increased expression of rhodopsin and its mRNA compared to the control groups. There is no difference between the defocus group and the control groups. Conclusion ： Expression of rhodopsin might involve formation of form-deprived myopia, but has less influence on defocus myopia. Eye Science 2008; 24： 1-5.