Polyhydroxybutyrate (哲学学士) 被加菌淤渣在一个二阶段的过程生产。在第一个阶段,有高存储潜力的污泥在动态喂政体操作的一个定序的批反应堆(SBR ) 被充实。哲学学士的生合成顺序在第二个阶段在一个批反应堆被完成。这篇文章由评估三个关键因素的效果集中于哲学学士内容的优化:在批反应堆的溶解的氧,酸碱值和 food-to-microorganism (F/M ) 比率。结果证明哲学学士的底层举起的率,以及收益和内容随溶解的氧集中的增加增加了。酸碱值的控制不在为哲学学士内容的改进的弱碱的条件下面是必要的,除了酸碱值价值是从的在盒子 7.0 ~ 10.0 响了。提高的 F/M 比率在 1.0 ~ 4.5 C-mmol/C-mmol 和最高的哲学学士内容的范围赞成了哲学学士累积, 64% ,在 4.5 C-mmol/C-mmol 被完成。当 F/M 比率被提高到 6.0 C-mmol/C-mmol 时,由污泥利用同时细胞内部的哲学学士和外部底层的现象被观察,它导致了哲学学士内容的锋利的减少。显微镜的观察清楚地证实那动态喂政体为与一个高存储潜力选择并且充实污泥是有效的。
Polyhydroxybutyrate (PHB) was produced by activated sludge in a two-stage process. In the first stage, the sludge with high storage potential was enriched in a sequencing batch reactor (SBR) operated by a dynamic feeding regime. The biosynthesis of PHB was sequentially accomplished in a batch reactor in the second stage. This article focused on the optimization of PHB content by evaluating the effects of three critical factors: Dissolved oxygen, pH and food-to-microorganism (F/M) ratio in the batch reactor. The results showed that the rate of substrate uptake, as well as the yield and content of PHB increased with the increase of dissolved oxygen concentration. The control of pH was not necessary under the weak alkaline condition for improvement of PHB content, except for the case in which the pH value was out of rang of 7.0 to 10.0. The enhanced F/M ratio favored PHB accumulation in the range of 1.0 to 4.5 C-mmol/C-mmol and the highest PHB content, 64%, was achieved at 4.5 C-mmol/C-mmoh When F/M ratio was enhanced to 6.0 C-mmol/C-mmol, the phenomenon of utilizing simultaneously intraceilular PHB and external substrate by sludge was observed, which resulted in a sharp decrease of PHB content. The microscopic observation clearly confirmed that dynamic feeding regime was effective for selecting and enriching the sludge with a high storage potential.