中文摘要：

通过酸化碳纳米管（CNTs）和β-环糊精（β-CD）之间的范德华力作用,实现CNTs的β-CD功能化.β-CD具有内腔疏水、外壁亲水的环状结构,其内腔容易与二茂铁（Fc）形成稳定的主客体包合结构,实现Fc在碳纳米管上的高效固载;再将CNTs-β-CD-Fc复合物与葡萄糖氧化酶（GOD）混合,采用戊二醛实现酶分子间的交联,形成GOD/CNTs-β-CD-Fc复合物,然后将其涂覆到玻碳电极（GC）上,得到一种新型的酶生物燃料电池阳极（GOD/CNTs-β-CD-Fc/GC）.采用同步热分析法、傅里叶变换红外光谱和透射电子显微镜对所制备的CNTs-β-CD-Fc复合物进行了表征,采用循环伏安法研究了GOD/CNTs-β-CD-Fc/GC电极对葡萄糖氧化的催化性能.结果表明：在同等实验条件下,没有固载Fc的GOD/CNTs-β-CD/GC电极基本无催化电流,而GOD/CNTs-β-CD-Fc/GC电极表现出比GOD/CNTs-Fc/GC电极更为优越的电催化性能.进一步以GOD/CNTs-β-CD-Fc/GC电极或GOD/CNTs-Fc/GC电极为酶阳极,商用催化剂E-TEK Pt/C电极（E-TEKPt/C/GC）为阴极,构建葡萄糖/氧气生物燃料电池（EBFC）,结果表明前者的最大功率密度（33μW cm-2,0.18 V）几乎是后者的三倍（11.7μW cm-2,0.16 V）.通过记录开路电位随时间的变化研究了EBFC的稳定性,以GOD/CNTs-β-CD-Fc/GC电极为阳极的EBFC在连续工作9 h后仍保留了92%的开路电位,表明该电池具有良好的连续工作稳定性.我们提出的这种新型生物燃料电池阳极的构造方法,为构建高性能、高稳定性的葡萄糖/氧气EBFC提供了新的思路.

英文摘要：

Acid-treated carbon nanotubes （CNTs） were functionalized by β-cyclodextrin （CD） through the van der Waals force between them. It is well-known that CD is toroidal in shape with a hydrophobic inner cavity and a hydrophilic exterior. Based on the recognition of CD to ferrocene （Fc）, a water-soluble complex, CNTs-β-CD-Fc, was prepared. Glucose oxidase （GOD） was mixed with CNTs-β-CD-Fc and crosslinked with glutaraldehyde to form enzyme polymer. Then the GOD/CNTs-β-CD-Fc composite was coated on the glassy carbon （GC） electrode and the GOD/CNTs-β-CD-Fc/GC bioanode was obtained. The prepared CNTs-β-CD and CNTs-fl-CD-Fc were characterized by thermal gravimeritric analysis, fourier transform infrared spectroscopy and transmission electron microscopy. The electrocatalytic properties of the GOD/CNTs-β-CD-Fc/GC bioanode towards glucose oxidation were investigated by cyclic voltammetry. The results showed that under the same experimental conditions, the GOD/CNTs-β-CD/GC electrode （without Fc） had almost no catalytic currents for glucose oxidation and the GOD/CNTs-fl-CD-Fc/GC bioanode had higher electrocatalytic activity towards glucose oxidation than the GOD/CNTs-Fc/GC electrode. The long-term cycle stability of the developed GOD/CNTs-β-CD-Fc/GC bioanode was also evaluated by cyclic voltammetry and the results showed that the GOD/CNTs-β-CD-Fc/GC bioanode had much better stability than the GOD/CNTs-Fc/GC electrode. Taking the commercial E-TEK Pt/C modified GC electrode as the cathode, the maximum power density of the glucose/O2 biofuel cell （EBFC） based on the GOD/CNTs-β-CD-Fc/GC anode was 33 μW·cm-2 （at 0. 18 V）, almost 3 times higher than that of the EBFC based on the GOD/CNTs-Fc/GC anode （11.7 μW·cm-2 at 0.16 V）. The stability of the developed EBFC was also investigated by monitoring the change of the open circuit potential （OCP） of EBFC. After continuous operation for 9 h, the developed EBFC （the GOD/CNTs-fl-CD-Fc/GC electrode as the bioanode） remained 92% of init