中文摘要：

目的探讨胃肠道间质瘤p16、Ki-67表达与临床病理的关系。方法制作40例胃肠道间质瘤组织芯片，并进行分组（低度危险组、中度危险组和高度危险组），以40例正常胃肠道肌壁组织为对照，用免疫组化Elivision二步法检测p16、Ki-67在胃肠道间质瘤组及对照组的表达。结果①Ki-67在胃肠间质瘤组中的表达高于在正常对照组中的表达，而p16的表达情况则正好相反，且差异有统计学意义（P〈0．032）；②p16、Ki-67在胃肠道间质瘤各组的表达无显著性差异；低、中度危险组合并后与高度危险组比较时未发现p16的表达差异（P〉0．05），而Ki-67则存在差异（P〈0．05）。结论①p16可能在胃肠道间质瘤的发生、发展中起重要作用；②Ki-67可作为评价肿瘤恶性潜能的一个重要指标；③应用组织芯片大规模高效检测临床组织样本是可行的，具有快速、方便、经济、准确的特点。

英文摘要：

Objective To investigate the relationship between the clinical pathological characteristic and the expressions of p16 and Ki-67 in gastrointestinal stromal tumor. Methods The tissue chips were made by using collected samples of 40 GIST and 40 normal control group, GIST was grouped into low-risk, intermediate-risk and high-risk, p16, Ki-67 were examined in every group by immunohistochemical method and the difference among those group was statisticed and analyzed respectively. Results ①The expressions of Ki-67 were higher in GIST group than that in normal control group, but the expression of p16 was on the contrary. And the differences had statistic significance（P〈0. 032）;②The expressions of Ki-67 and p16 in low-risk, intermediaterisk and high-risk GIST groups had no significant differences; while Ki-67 showed significant difference between combined group of low-risk with intermediate-risk and high-risk group（P〈0.05）,but p16 didn＇t show any difference in the adjusted group. Conclusion ① p16 may play an important role in the pathogenesis of GIST; ②Ki-67 can be regarded as an important parameter in judging the risk of GIST. ③It is feasible to utilize tissue chip for a rapid, economic and accurate screening of clinical tissue specimens on a large scale.