中文摘要：

建立了一种采用KYA HIQ sil C18柱（250mm×4．6mm，5μm），以乙腈-水溶液（体积比1：9）为流动相，在流速1．0mL／min、检测波长280nm、进样量10μL的条件下，用高效液相色谱同时测定红景天苷及其苷元酪醇含量的方法。利用均匀试验设计研究了分析样品的制备方法，确定了红景天根粉的最佳提取条件为：以蒸馏水为溶剂在60℃下超声波提取50min。分别测定了不同产地的红景天属植物中红景天苷和酪醇的含量，西藏产圣地红景天中红景天苷含量最高，达到2．147％，其次为藏产大花红景天和西藏红景天，分别为1．763％和1．271％；四川产大花红景天中酪醇含量最高，为0．2985％，其次是藏产西藏红景天和大花红景天，分别为0．2143％和0．1836％；长鞭红景天和狭叶红景天中2种有效成分的含量均很低，红景天苷含量仅为0．034％和0．003％，酪醇含量则分别为0．0164％和0．0048％。

英文摘要：

A method for the determination of contents of salidroside and tyrosol in Rhodiola by HPLC was established. The HPLC separation was preformed on a KYA HIQ sil C18 column （250 mm×4.6 mm,5 μm）, at flow rate 1 mL/min,with mobile phase of acetonitrile/water （ 1 ： 9） and detected at wavelength 280 nm. Using uniform design, the optimal conditions for salidroside and tyrosol extraction were explored to be ultrasonically extracted by distilled water at 60 ℃ for 50 min. Contents of salidroside and tyrosol in Rhodiola roots were determined. Salidroside content was 2. 147 % , the highest in Rh. sacra from Tibet, then in Rh. crenulata and Rh. tibetica from Tibet, 1. 763 % and 1. 271%, respectively; tyrosol content was 0.298 5 % ,the highest in Rh. crenulata from Sichuan, then in Rh. tibetica and Rh. crenulata from Tibet, 0. 214 3 % and 0. 183 6 %, respectively; whereas both contents were very low in Rh. fastigiata and Rh. kirilowii from Sichuan with salidroside contents of only 0.034 % and 0.003 %, and tyrosol contents of 0.016 4 % and 0.004 8 %, respectively.