中文摘要：

对浙江省杭州地区白菜（Brassica campestris L．ssp．chinensis vat．commuis Tsen et Lee．）上获得的CMV分离物（CMV-CTL）进行了全长克隆和基因组序列分析。结果显示：CMV—CTL的RNA1（Gen—Bank序列号：EF213023）全长为3357个核苷酸（nt），编码993个氨基酸（aa）的1a蛋白；RNA2（Gen-Bank序列号：EF213024）全长为3047nt，编码858aa的2a蛋白和111aa的2b蛋白；RNA3（GenBank序列号：EF213025）全长为2217nt，编码278aa的MP蛋白和218aa的CP蛋白。序列相似性分析表明，CMV-CTL与CMV亚组B中株系IA相似性最高，RNA1、RNA2和RNA3与该株系的相似性分别为91．3％、91．3％和93．6％。CP基因和RNA3的5′NTR核酸序列系统发生树分析表明，CMV—CTL与中国大多数CMV分离物一样，属于CMV亚组IB。

英文摘要：

Isolate CMV-CTL was obtained from Brassica campestris L. ssp. chinensis var. commuis Tsen et lee. , in Hangzhou, Zhejiang Province. The full-length cDNA sequence of genomic RNAs of the isolate was determined by RT-PCR and sequencing. The results showed that CMV-CTL RNA1 （GenBank accession No. EF213023 ） consists of 3 357 nucleotides （nt）, encoding 1a protein of 993 amino acids residues （aa）. RNA2 （GenBank accession No. EF213024） consists of 3 047 nt, encoding 2a protein of 858 aa and 2b protein of 111 aa. RNA3 （GenBank accession No. EF213025） contains of 2 217 nt, encoding 3a protein of 278 aa and CP of 218 aa. CMV-CTL showed the highest sequence identities with strain IA which belongs to CMV sub- group IB. The sequence identities of RNA1, RNA2 and RNA3 between CTL and IA strains were 91.3%, 91.3% and 93.6%, respectively. Phylogenetic analysis of CP and 5′NTR of RNA3 suggested that CMV-CTL belongs to CMV subgroup IB.