中文摘要：

卫星 RNA (satRNAs ) 是防碍助手病毒的致病的分子的寄生虫。在这研究，黄瓜马赛克的相对累积病毒(CMV )-Fny genomic RNA 与或没有 satRNAs 是 real-timeRT-PCR 分析的份量上。结果证明 satRs 显然由压抑 CMV-Fny genomic RNA 的累积在 Nicotianatabacum 上稀释了 CMV-Fny 的症状，测试同样开的读物框架。CMV-Fny 1a， 2a， 2b， 3a，和 CP 基因的 Theaccumulation 比有 satRsadded (CMV-Fsat ) 的 CMV-Fny 的高得多，在不同接种时间。 CMV-Fny Δ2 b ，在 2a 基因的C终端的完全的 2b 基因和 41amino 酸在被删除，在 N.tabacum 幼苗上引起了仅仅细微马赛克效果，类似于 CMV-Fsat 的，但是到 CMV-Fny Δ2 b 的 satRs 的增加在 CMV-Fny Δ2 b genomic RNA 的累积显示出进一步的减少。我们的结果显示由增加 satRs 或删除 2b 基因， CMV 的变细由于 CMVgenomic RNA，和在 N 的 CMV genomic RNA 累积的那调停 satRNA 的减小的低累积。tabacum 是可能与 2b 基因有关。

英文摘要：

Satellite RNAs （satRNAs） are molecular parasites that interfere with the pathogenesis of the helper viruses. In this study, the relative accumulation of cucumber mosaic virus （CMV）-Fny genomic RNAs with or without satRNAs were quantitatively analyzed by real-time RT-PCR. The results showed that satRs apparently attenuated the symptoms of CMV-Fny on Nicotiana tabacum by depressing the accumulation of CMV-Fny genomic RNAs, tested as open reading frames. The accumulation of CMV-Fny 1a, 2a, 2b, 3a, and CP genes was much higher than that of CMV-Fny with satRs added （CMV-Fsat）, at different inoculation times. CMV-Fny△2b, in which the complete 2b gene and 41 amino acids at the C-terminal of the 2a gene were deleted, caused only a slight mosaic effect on N. tabacum seedlings, similar to that of CMVFsat, but the addition of satRs to CMV-Fny△2b showed further decrease in the accumulation of CMV- Fny△2b genomic RNAs. Our results indicated that the attenuation of CMV, by adding satRs or deleting the 2b gene, was due to the low accumulation of CMV genomic RNAs, and that satRNA-mediated reduction of CMV genomic RNAs accumulation in N. tabacum was possibly related to the 2b gene.