中文摘要：

[目的]观察重症急性胰腺炎（SAP）大鼠胰腺组织腺苷酸环化酶（AC）活性的变化及银杏苦内酯BN52021）的影响。[方法]Wistar雄性大鼠随机分为阴性对照组（NC组）、SAP模型组（SAP）和BN52021治疗组（BN组），每组按术后不同时相点（1、2、3、6、12、24h）分为6小组，用放射免疫的方法检测胰腺组织中AC活性的变化，同时对胰腺组织进行病理学观察和测定血清淀粉酶的变化。[结果]血清淀粉酶和病理学结果显示SAP大鼠制模成功，BN52021能降低SAP的血清淀粉酶和改善病理学变化；SAP组AC活性在2h，3h较Nc组显著升高（P〈0．05）；BN组AC活性在2、3、12h较NC组显著升高（P〈0．05）；在2h、3h较SAP组显著降低（P〈0．05）。[结论]SAP发生后，胰腺组织AC活性升高，可能通过第二信使途径完成细胞内信号转导，导致炎症反应延续并放大。BN52021通过降低SAP大鼠胰腺组织AC活性发挥治疗作用。

英文摘要：

[ Objective] To observe the activity of adenylate cyclase （AC） in severe acute pancreatitis （SAP） of rat treated with ginkgolide B （BN52021） . [Methods] Male Wistar rats were randomly divided into 3 groups： negative control group （NC group）, SAP model group（SAP group）and BN52021 treated group （BN group） .Rats were killed after operation and at 1, 2, 3, 6, 12, 24 h after treatment. The activity of AC in pancreatic tissues were measured with radio-immunity method, Serum amylase and pancreatic pathological changes were observed in three groups. [ Results] Serum amylase and pancreatic pathology showed that the SAP model was successful prepared and BN52021 had therapeutic effect on SAP. The AC activity in SAP group was significant increased as compared with NC groups at 2 h and 3 h （ P 〈 0.05）, The AC activity in BN group showed a significant increasas compared with in NC groups at 2 h, 3 h and 12 h（ P 〈 0.05）, and the activity in BN group showed a aignifican decrease compared with NC group at 2 h, 3 h（ P 〈 0.05）. [ Conclusions] The activity of AC is remarkably increased in SAP rat and may be correlated with the intracellular singal transduction by second messenger. BN52021 can decrease the activaliy of AC in pancreatic tissue and it provides amolecular mechanism for the treatment on SAP.