中文摘要：

目的研究3种常用铸造合金全冠戴用后Ki67、P53蛋白在牙龈组织中的表达,探讨3种常用牙科铸造合金的生物学性能。方法对犬分别行58%金合金、不含铍的镍铬合金、含铍的镍铬合金铸造全冠修复,建立实验动物模型。应用免疫组化Envision二步法检测戴冠前、戴冠后2周,1、2及3个月时Ki67、P53蛋白在牙龈组织中的表达。结果含铍的镍铬合金与不含铍的镍铬合金铸造全冠戴用后,Ki67、P53蛋白在牙龈组织的表达逐渐升高,戴冠1个月后达到最高,随后逐渐降低;而58%金合金全冠戴用后,Ki67、P53蛋白的阳性表达率与空白对照组无统计学差异,随时间延长也无明显改变。结论镍铬合金全冠戴用后,牙龈组织中Ki67、P53蛋白表达明显,而58%金合金全冠戴用后表达不明显。3种铸造合金材料均未造成长时期的牙龈上皮异常增殖。

英文摘要：

Objective The aim of the study was to detect the expression of Ki67 and P53 proteins in the gingival tissue before and after wearing three kinds of dental casting alloy crowns.Methods The three kinds of dental casting alloys（58% golden alloy,NiCr alloy,NiCr alloy excluding Be） were used to make full crowns for 3 dogs in each group.Another 3 dogs were taken as control.The immunohistochemistry Envision method was used to measure the expression of Ki67 and P53 proteins in the gingival tissue before wearing the crowns,2 weeks,1 month,2 months,3 months after wearing the crowns.Results The expression of Ki67 and P53 proteins in gingival tissue increased 1 month after wearing NiCr alloy and NiCr alloy excluding Be,then decreased gradually.And the expression of Ki67 and P53 proteins in 58% golden alloy group was similar to that in control.No pathological change was observed in all samples.Conclusion Two kinds of NiCr alloy crowns both can increase the expression of Ki67 and P53 proteins in gingival tissue,but 58% golden alloy crowns can not.So compared with NiCr alloy crowns,58% golden alloy crowns have better biological properties.And none of three kinds of dental alloy crowns may induce long-term abnormal proliferation in gingival tissue.