中文摘要：

目的研究表没食子儿茶素没食子酸酯（epigallocatechin gallate,EGCG）及镍离子（Ni2＋）共同作用对舌鳞癌细胞系Cal-27 cyclinD1和survivin基因表达的影响。方法 MTT法检测Cal-27的细胞存活率;real-time PCR法检测cyclinD1及survivin mRNA水平表达的影响;Western blot法检测cyclinD1和survivin蛋白水平的表达情况。结果高浓度EGCG（〉100μmol/L）可明显抑制Cal-27生长,且对细胞cyclinD1和survivin基因转录及蛋白表达有明显下调作用（P〈0.05）;Ni2＋与EGCG共同作用增加了对Cal-27的抑制作用,cyclinD1和survivin表达更加显著下降（P〈0.05）。结论 Ni2＋与EGCG共同作用后可增强EGCG对细胞生存率及cyclinD1和survivin基因表达的抑制作用,两者起协同作用。

英文摘要：

Objective To determine the effect of epigallocatechin gallate（EGCG） and Ni2＋ on the expression of cyclinD1 and survivin in tongue squamous carcinoma cells（Cal-27）. Methods The viability of cells was determined by the MTT reduction assay. Real-time PCR was used to examine the mRNA expression of cyclinD1 and survivin. Western blot method was used to analyze the expression level of protein of cyclinDl and sl~rvivin. Results The proliferation of Cal-27 cells was significantly inhibited by high concentration of EGCG （greater than 100 μmol/L）. The mRNA expression and protein expression of cyclinD1 and survivin were also downregulated （P〈0. 05）. Ni2＋ could enhance the inhibition of Cal-27 cells by EGCG. The expression of cyclinDl and survivill were significantly suppressed by EGCG plus Ni2＋ （P〈0.05）. Conclusions The inhibition of Cal-27 cells and expression of cyclinDl and survivin by EGCG could be enhanced by the mixture of Ni2＋ .