中文摘要：

目的 用人肝肿瘤细胞HepG2／体外微核试验检测3种持久性有机污染物（POPs）Aroclor1254、毒杀芬和滴滴涕的遗传毒性。方法 人肝肿瘤细胞HepG2经Aroclor1254、毒杀芬和滴滴涕染毒24h，继续在补充细胞松弛素B（3μg/ml）的培养液中培养24h后，计数1000个双核细胞中的微核。结果 20，40μmol／L毒杀芬处理HepG2细胞的微核率与溶剂对照相比显著增加（P〈0.01，P〈0．01）；经Aroclor1254（23～184μmol／L）和滴滴涕（17．8～60μmol／L）处理的HepG2细胞的微核率与溶剂对照相比，差异无统计学意义。结论 Arodor1254和滴滴涕未显示对HepG2细胞明显的遗传毒性；毒杀芬可诱导HepG2细胞遗传损伤，有必要进一步评价其对人类健康的潜在危害。

英文摘要：

Objective To investigate genetic toxicity of three persistent organic pollutants （POPs）, including aroclor1254, DDT and Toxaphene, using human derived hepatoma（HepG2）cells/in vitro micronucleus assay. Methods Hep G2 cells were treated with Aroelor1254, DDT and Toxaphene respectively for 24 hours, and further incubated in the medium supplemented with cytochalasin B（3 μg/ml）for 24 hours. Micronuclei（MN） were scored in 1 000 binucleated Hep G2 cells. Resuits Compared with solvent control, toxaphene increased significantly MN frequencies in Hep G2 cells at concentrations of 20,40 μmol/L respectively（P 〈 0.01, P 〈 0.01）. No significant increase of MN frequencies were found in Hep G2 cells treated with Aroclor1254（23- 184 μmol/L） and DDT（ 17.8-60 μmol/L）. Conclusion Aroelor1254 and DDT do not show clear genetic toxicity in Hep G2 cells. Toxaphene could induce genetic damage in Hep G2 cells, and further study is needed to evaluate its potential adverse effects on human health.