中文摘要：

背景与目的：探讨2，2′，4，4′-四溴联苯醚（2，2′，4，414etrabromodiphenyl ethers，PBDE-47）对人神经母细胞瘤SH-SY5Y细胞的致突变作用。材料与方法：设空白对照组、溶剂对照组、3个不同BDE-47剂量的试验组（1μg、2μg、4μg／m1）和阳性对照组（MMC），共6组。SH-SY5Y细胞分别暴露于各组不同受试物24h后，采用胞质分裂阻断法测定微核率、核浆桥率。结果：PBDE-47可诱导SH-SY5Y细胞核分裂指数呈剂量依赖性下降，微核细胞率和核浆桥率呈剂量依赖性增加；2μg／ml和4μg／ml的染毒剂量可引起SH-SYSY细胞核分裂指数明显降低（P〈0．05）以及微核细胞率和核浆桥率的明显增加（P〈0．05），而仅在4μg/mlPBDE-47染毒组发现微核率明显增加（P〈0．05）。结论：PBDE-47可诱导SH-SY5Y细胞微核和核浆桥的形成，具有致突变作用。

英文摘要：

BACKGROUND ＆ AIM： To study the mutagenicity of 2, 2′ , 4, 4′-tetrabromodiphenyl ethers （PBDE-47） in human neuroblastoma SH-SY5Y cells in vitro. MATERIALS AND METHODS： The frequencies of mieronuelei（MNi） and nueleoplasmie bridges （NPBs） were measured after SH-SY5Y cells were exposed to different doses of PBDE-47 for 24 h in vitro. RESULTS： PBDE-47 caused a significant concentration-dependent decrease in nuclear division index（NDI）, and a concentration-dependent increase in MNi frequency in terms of total MNi/ 1000 binueleated cells （BNCs）, BNCs with MNi/1000 BNCs, and NPBs/1000 BNCs. The NDI was obviously decreased, while the frequencies of BNCs with MNi/ 1000 BNCs and NPBs/1000 BNCs were increased significantly at 2 μg/ ml and above PBDE-47 -treated groups （P 〈 0.05）. There was statistically difference in frequeeney of MNi/1000 BNCs only in 4 μg/ml PBDE-47-treated group （P〈0.05）. CONCLUSION： PBDE-47 may induce the MNi and NPB formation, causing mutagenieity.