中文摘要：

为了探索UBA52基因表达规律与山羊性早熟的关系，本研究以性早熟品种济宁青山羊与性晚熟品种辽宁绒山羊为研究对象，分别应用RT—PCR与荧光定量PCR技术检测UBA52基因在这2种山羊不同发育阶段的组织表达情况和下丘脑、垂体、卵巢、子宫、肾上腺5种组织中的mRNA表达水平。结果显示，UBA52基因在2种山羊各发育阶段各组织中广泛表达，并在子宫中的表达量最高，同时济宁青山羊垂体中UBA52基因表达量在初情期时极显著低于幼年期（P〈0．01），而辽宁绒山羊UBA52基因表达量在幼年期Ⅱ时极显著高于幼年期Ⅰ，初情期时则极显著低于幼年期Ⅱ（P〈0．01）。本研究结果表明UBA52基因表达上调与山羊发情呈负相关，推测UBA52表达上调可能通过使得促卵泡素（FSH）和促黄体素（LH）的分泌量减少来抑制山羊初情期的发生。

英文摘要：

In order to detect the association of UBA52 gene and sexual precocity in goats, female sexual precocious goat breed Jining Grey goat and female sexual late-maturing goat breed Liaon- ing Cashmere goat were selected. RT-PCR method was used to detect the expression profile of UBA52 gene in tissues at different developmental stages of the two breeds, and then real-time PCR technology was used to exam the mRNA expression level of UBA52 genes in hypothalamus, pituitary gland, ovary, uterus and adrenal gland. The result showed that UBA52 gene was widely expressed in the organizations at different developmental stages of the two breeds, and its expression level in the uterus was the highest. The results also showed that the expression level of UBA52 in pituitary gland in puberty was significantly lower than that in juvenile period of Jining Grey goat （P〈0.01）, while the expression level in juvenile period II was significantly higher than that in juvenile period I of Liaoning Cashmere goat, and its expression level in puberty was significantly lower than that in juvenile period II （P〈0. 01）. The results indicated that the up-regulatedexpression of UBA52 gene was negatively correlated to goats estrus. So it was concluded that the up-regulation of UBA52 may reduce the secretion of follicle-stimulating hormone （FSH） and lute- inizing hormone （LH） to suppress the occurrence of goat puberty.