中文摘要：

目的：探讨偏头痛大鼠模型颈髓1-2中间神经元的变化及其对运动神经元的影响。方法：雄性SD大鼠随机分为空白组、假手术组、致炎剂（inflammatory soup,IS）组（IS 3 d、6 d、10 d）、生理盐水组。采用IS刺激大鼠硬脑膜模型,应用Von Frey纤维丝测定大鼠眶周痛觉阈值,应用Western blot和免疫荧光法观察颈髓中间神经元、运动神经元的变化。结果：随着IS反复刺激,大鼠眶周痛阈逐渐下降;IS组大鼠运动神经元乙酰胆碱转移酶Ch AT和c-Fos表达量较对照组增多,平均吸光度值增加;谷氨酸能中间神经元表达增强,GABA能中间神经元表达减弱。结论：反复硬脑膜给予致炎剂能有效诱导大鼠反复发作偏头痛并出现痛觉超敏,颈髓前角运动神经元兴奋性增加,这可能与颈髓中间神经元＂兴奋—抑制＂稳态失调相关。

英文摘要：

Objective： To explore the changes of interneurons and their effects on motor neurons in the upper cervical spinal cord of a rat migraine model. Methods： Male SD rats were randomly divided into： naive group, sham surgery group, inflammatory soup（IS 3 d, 6 d, 10 d） group and saline group. Following IS stimulation in the rat dual matter, Von Frey hair was used to test the pain threshold of periorbital skin. Western blot and immunofluorescence techniques were used to detect possible changes of excitatory and inhibitory interneurons and expression of choline acetyltransferase（Ch AT） and c-Fos proteins in the upper cervical spinal motor neurons. Results： Compared with control groups, rats in IS groups exhibited mechanical allodynia. The protein expression and mean optical density（OD） of Ch AT and c-Fos in the upper cervical spinal motor neurons were significantly increased. The mean OD of phosphate-activated glutaminase（PAG） immunoreactivity was increased, while the mean OD of γ-aminobutyric acid（GABA） immunoreactivity was decreased, changes in both groups were statistically significant. Conclusion： Prolonged dural inflammation can induce allodynia in rat model of migraine and promote activation of motor neurons in the upper cervical spinal anterior horn. The possible mechanism may be the imbalance of excitatory and inhibitory interneuron modulation.