中文摘要：

目的探讨托吡酯对偏头痛模型大鼠三叉神经节内核苷酸结合寡聚化结构域样受体3（NALP3）炎性小体及白介素-1β（IL-1β）表达的影响。方法40只雄性成年SD大鼠按随机数字表法分为8组：空白组、生理盐水组、模型组、预防对照组、10 mg/kg托吡酯组、30 mg/kg托吡酯组、60 mg/kg托吡酯组和90 mg/kg托吡酯组，每组5只。采用致炎剂反复刺激硬脑膜法建立偏头痛模型，其中空白组不做任何处理，生理盐水组给予生理盐水刺激硬脑膜，不同浓度托吡酯组给予模型大鼠不同浓度托吡酯处理，预防对照组给予模型大鼠含1 %Tween80的生理盐水处理。最后一次给药后3 h，采用Western blotting检测各组大鼠三叉神经节内NALP3、胱天蛋白酶1前体（pro-Caspase-1）、胱天蛋白酶1（Caspase-1）、IL-1β的表达量，并选择出最佳托吡酯浓度行后续实验。10只雄性成年SD大鼠按随机数字表法分为对照组（与预防对照组干预相同）和托吡酯组（与60 mg/kg托吡酯组干预相同），每组5只。采用免疫荧光染色观察三叉神经节内NALP3、Caspase-1、IL-1β的表达情况。结果与空白组比较，生理盐水组NALP3、pro-Caspase-1、Caspase-1、IL-1β表达量差异均无统计学意义（P〉 0.05）；与生理盐水组比较，模型组NALP3、pro-Caspase-1、Caspase-1、IL-1β表达量明显增加，差异均有统计学意义（P〈0.05）；与预防对照组比较，60 mg/kg托吡酯组、90 mg/kg托吡酯组NALP3、pro-Caspase-1、Caspase-1、IL-1β表达量明显降低，差异均有统计学意义（P〈0.05）。与对照组比较，托吡酯组NALP3、Caspase-1、IL-1β免疫荧光染色强度明显减弱。结论托吡酯能够抑制偏头痛模型大鼠三叉神经节内NALP3炎性小体和IL-1β表达，其可能是偏头痛预防治疗的重要机制之一。

英文摘要：

Objective To investigate the effect oftopiramate on NACHT-LRR-PYD-containing protein 3 （NALP3） inflammasome and interleukin （IL）-1β levels in trigeminal ganglion of migraine rats. Methods Forty adult male Sprague Dawley rats were randomly divided into blank group, saline group, model group, prevention control group, 10 mg/kg topiramate group, 30 mg/kg topiramate group, 60 mg/kg topiramate group, and 90 mg/kg topiramate group （n=5）. Inflammatory soup was used to stimulate the dual matter of rats repeatedly to induce migraine models： rats in the blank group were without any treatment, those in the saline group were given saline to stimulate the dual matter, different concentrationsof topiramate group were given to migraine models of the 10 mg/kg topiramate group, 30 mg/kg topiramate group, 60 mg/kg topiramate group, and 90 mg/kg topiramate group, respectively, and migraine models of the prevention control group were given saline containing 1% Tween80. Three h after the last treatment, the expressions of NALP3, caspase-1 precursor （pro-caspase-1）, caspase-1 and IL-1β in trigeminal ganglion of rats were detected by Westem blotting, and the best concentration of topiramate was chosen for subsequent immunofluorescence experiments. Ten healthy male adult SD rats were randomly divided into control group and topiramate group （n=5）; the expression levels of NALP3, caspase-1 and IL-1β in trigeminal ganglion of the two groups were detected by immunofluorescence. Results The expression levels ofNALP3, pro-caspase-1, caspase-1 and IL-1β were not significantly different between saline group and blank group （/9〉0.05）; the expression levels ofNALP3, pro-caspase-1, caspase-1 and IL-1β in the model group were significantly higher than those in the saline group （P〈0.05）. The expression levels ofNALP3, pro-caspase-1, caspase-1 and IL-1β in 60 mg/kg topiramate group and 90 mg/kg topiramate group were significantly lower than those in the prevention control group （P〈0.05）. The fluorescence