中文摘要：

[目的]建立从冷冻血凝块中提取基因组DNA的改良方法．[方法]取冷冻血凝块在4℃条件下解冻，经高盐及高EDTA提取液处理，消化液消化，异丙醇抽提．[结果]用改良异丙醇法成功地从人血凝块中提取基因组DNA，其质量浓度为（22．5±7．2）mg／L，光吸收比值A260／A280为1．63～2．00．[结论]应用改良法提取的基因组DNA的总量较多，提取率高，PCR扩增效果较好，可用于血凝块基因组DNA的提取．

英文摘要：

OBJECTIVE To explore a simple method of DNA extraction from human blood clots. METHODS Blood clot was thawed at 4℃, homogenized extraction buffer including higher sodium chloride and higher EDTA, the homogenized samples were digested with digestion buffer and extracted with phenol-chloroform. RESULTS The genomic DNA was successfully extracted. The concentration of the extracted DNA was （22.5 % 7.2） mg/L, and OD value A260/280 was 1.63 % 2.00. CONCLUSION The improved extraction method is reliable for obtaining high quantities of DNA from blood clot and suites for PCR amplification.