中文摘要：

研究目的：创新要点：通过分析miRNA的核心启动子和顺式作用元件为进一步解析大豆（Glycinemax）miRNAs表达调控及其功能研究提供重要信息。利用生物信息学方法全面解析了大豆降解组文库miRNA的启动子特征，并依据顺式作用元件及靶基因构建了miRNA的表达与生长素响应因子、赤霉素响应因子之问存在潜在的负反馈调控网络。研究方法：本研究利用TSSP程序和PlantCARE数据库预测了来自大豆降解组文库的440个miRNA的核心启动子以及369个miRNAs的顺式作用元件，并依据顺式作用元件及靶基因构建miRNA调控网络。重要结论：83．86％的miRNA在其上游序列中含有启动子，8．64％的miRNA在其下游序列中含有启动子，21．59％的miRNA包含增强子。核心启动子的TATA盒与转录起始位点（TSSs）的分布相似（见图2）。此外，对转录起始位点5’端的顺式作用元件预测为miRNAs的可能功能和表达的时空性提供了线索。miRNAs的顺式作用元件和靶基因的分析显示，部分miRNA的表达与生长素响应因子、赤霉素响应因子之间存在潜在的负反馈调控（见图3）。

英文摘要：

Objective： MicroRNAs （miRNAs） are genome-encoded, small non-coding RNAs that play important functions in development, biotic and abiotic stress responses, and other processes. Our aim was to explore the regu- lation of miRNA expression. Methods： We used bioinformatics methods to predict the core promoters of 440 miRNAs identified from a soybean （Glycine max） degradome library and to analyze c/s-acting elements for 369 miRNAs. Results： The prediction results showed that 83.86% of the 440 miRNAs contained promoters in their upstream se- quences, and 8.64% （38 loci） in their downstream sequences. The distributions of two core promoter elements, TATA-boxes and transcription start sites （TSSs）, were similar. The cis-acUng elements were examined to provide clues to the function and regulation of spatiotemporal expression of the miRNAs. Analyses of miRNA cis-elements and targets indicated a potential auxin response factor （ARF）- and gibberellin response factor （GARF）-mediated negative feedback loop for miRNA expression. Conclusions： The features of miRNAs from a Glycine max degradome library obtained here provide insights into the transcription regulation and functions of miRNAs in soybean.