采用峰值场强为100 kV·m^-1、脉宽80 ms、脉冲频率为1 Hz的极低频高压脉冲电场(ELF-PEF )处理-0.1 MPa的PEG -6000溶液中萌发的绿豆种子,研究了绿豆在萌发过程中鲜重、自发发光、延迟发光、丙二醛(MDA )含量和超氧化物歧化酶(SOD )活性的变化。结果表明,ELF-PEF对PEG胁迫下萌发的绿豆鲜重、自发发光和延迟发光积分强度促进作用分别达到了28.58%、78.35%和40.6%,说明ELF-PEF可以有效促进绿豆种子萌发过程中的生理代谢,缓解干旱胁迫对细胞的伤害作用。ELF-PEF通过提高PEG胁迫下萌发绿豆中SOD活性,降低MDA含量,缓解干旱胁迫对萌发绿豆的氧化损伤,ELF-PEF对细胞毛细吸水和代谢吸水的促进可能是其缓解干旱伤害的原因。
Mung beans soaked in -0 .1MPa PEG solution were treated by extremely low frequency pulsed electric field (ELF-PEF)with peak electric field strength of 100 kV·m^-1 ,pulse width of 80 ms ,and pulse frequency of 1 Hz . Traits like fresh weight ,spontaneous luminescence ,delayed luminescence ,malondialdehyde (MDA ) and superoxide dis-mutase (SOD) activity during mung beans germination were investigated .The fresh weight ,spontaneous luminescence and delayed luminescence integral intensity in germinating mung beans were 28 .58% ,78 .35% and 40 .6% ,respec-tively under PEG stress ,indicating that ELF-PEF can accelerate the physiological metabolismand alleviate cells injury under drought stress . Although with increased SOD activity and simultaneously reducing MDA content , the oxidation damage caused by drought stress was alleviated by ELF-PEF in germinating mung beans .The promoting effect of ELF-PEF on capillary water absorption and metabolism water absorption in cells may be the cause of alleviating drought injury .