为开展极低频高压脉冲电场生物学效应的研究,设计了基于植物本征电位波动的极低频高压脉冲电场生物学效应仪,该系统可以产生幅值高压在6~20kV、输出频率在0.1~15Hz范围内数字式可调的极低频、宽脉宽、上升前沿陡的高压脉冲。用100kV/m、1Hz、80ms脉宽的极低频高压脉冲电场处理萌发中的绿豆和玉米种子,发现极低频高压脉冲电场对绿豆和玉米种子的发芽和生长具有较强的促进作用,说明基于植物电位波动的极低频高压脉冲电场具有明显的生物学效应,研究结果可为极低频高压脉冲电场生物学效应的深入研究提供参考。
In order to carry out the study of biological effects on extremely low frequency high-voltage pulsed electric field,a pulsed electric field generator based on potential fluctuation in plant was designed.The system can produce high-voltage pulse with extremely low frequency,wide pulse width and steep front.The amplitude of voltage is adjustable from 6 to 20 kV and the pulse frequency is adjustable from 0.1 to 15 Hz.Using the pulsed electric field with 100 kV/m,1 Hz and 80 ms pulse width to treat germinating mung beans and maize seeds,the results indicated that the shoot length,root length and root number of mung beans and maize were significantly promoted.It was illustrated that the extremely low frequency high-voltage pulsed electric field based on potential fluctuations in plant had a significant biological effects.This paper laid the foundation for further study of biological effect on extremely low-frequency high-voltage pulsed electric field.