为了获取显著的电场生物学效应,通过小波降噪和功率谱分析研究了玉米幼苗本征电位波动的基本特征。研究发现,玉米幼苗本征电位波动的功率谱主要分布在1Hz以内,重心频率为0.2Hz。采用电场强度为100kV/m、频率为0.2Hz的极低频脉冲电场处理萌发玉米种子,在萌发第5d时玉米种子的质量、芽长和根长分别比对照组增加17.55%、60.13%和28.50%。分析萌发种子超弱发光的变化时发现,在萌发第5d时,处理组玉米种子的自发发光和延迟发光积分强度分别比对照组增加了68.84%和33.93%,表明0.2Hz脉冲电场加速了玉米萌发过程中DNA合成反应和细胞代谢。脉冲电场与植物电位的耦合共振可能是极低频脉冲电场具有显著生物学效应的原因。
In order to obtain remarkable electric field biological effect on crop seeds,the basic characteristics of natural potential fluctuations in maize seedlings were studied by analyzing the wavelet de-noising and power spectrum.The results showed that the power spectrum of natural potential fluctuations in maize seedlings was mainly distributed in 0-1 Hz with a gravity frequency of 0.2 Hz.Based on the potential fluctuation characteristics,extremely low frequency pulsed electric field of 100 kV/m and 0.2 Hz was used to treat germinating maize seeds.It was found that the seed germination process was significantly promoted: on the 5th day of germination,the fresh mass,shoot length and root length of germinating maize seed respectively increased by 17.55%,60.13% and 28.50% compared with those of the control group.Analysis results of ultraweak luminescence(UL) changes in the geminating seeds showed that the ultraweak luminescence was promoted by the pulsed electric field: the spontaneous luminescence and the integrated intensity of delayed luminescence of the germinating maize seed respectively increased by 68.84% and 33.93% compared with those of the control group on the 5th day of germination.The promotion implied that the 0.2 Hz extremely low frequency pulsed electric field accelerated the DNA synthesis reaction and cell metabolism of the germinating seeds.It is concluded that the coupled resonance between pulsed electric field and plant natural potential may be the cause of the extremely low frequency pulsed electric field's biological effects.