中文摘要：

实验旨在研究蛋氨酸脑啡肽（MENK）对禽马立克细胞MSB—1生长抑制作用和凋亡诱导效应。用MENK对禽马立克细胞MSB-1细胞株进行干预，采用光镜观察细胞密度及形态，MTT法测定细胞增殖抑制率，流式细胞术检测细胞凋亡。镜下观察可见在10^-12mol/l MENK的作用下，MSB-1细胞株数量降低，细胞形态渐圆钝，甚至出现皱缩，与对照组相比MENK对MSB-1细胞具有明显的生长抑制作用，抑制率为19％-46％fP〈0．01），诱导细胞凋亡率为（62．04±3．32）％（P〈0．01）。MENK对MSB-1细胞株具有增殖抑制效应及凋亡诱导效应，是具有发展潜力的抗肿瘤药物。

英文摘要：

The purpose of the test was to investigate the effects of MENK on cell proliferation and apotosis in MSB-1 cells. The cultured MSB-1 cells were treated with MENK.Optical micro- scope was used to observe the morphology, the proliferation of MSB-1 cells was detected by MTT method.Cell apeptosis was detected by flow cytometry. MSB-1 cells gradually became blunt, or even shrinked treated with 10^-12 mol/1 MENK. MENK inhibited the proliferation of MSB-1 cells compared with control group, the cell growth inhibition rate was 19%-46%（P〈 0.01）, and the apoptosis rate was（62.04±3.32）%（P〈0.01）. MENK induced the apoptosis and in- hibited the cell proliferation of MSB-1, which was a potential antitumor drugs.