中文摘要：

观察蛋氨酸脑啡肽（MENK）对巨噬细胞一粒细胞集落刺激因子（GM-CSF）诱导的小鼠骨髓来源树突状细胞（DC）成熟及TILR-4表达的影响，探讨蛋氨酸脑啡肽对小鼠骨髓来源DCs免疫功能的调节机制。体外制备小鼠骨髓细胞用GM—CSF协同MENK诱导培养7d后用RT—PCR法检测TLR-4的mRNA表达，用western-blot检测TLR-4蛋白的表达，同时用流式细胞仪（FACS）分析DC表型特征，混合淋巴细胞反应（MILR）检测DC的功能。结果显示MENK协同诱导组树突状细胞TLR-4和共刺激分子的表达高于正常对照组（P〈0．01）和GMCSF诱导组（P〈0．05）并具有强的激发MLR的能力。提示MENK可以促进DC成熟及TLR-4的表达，增强DC免疫功能。

英文摘要：

To observe the effect of methionine enkephalin（MENK） on the maturation and expression of Toll like receptor 4 （TLR-4） of DCs derived from murine bone marrow progenitors（BM-DCs）induced by GM-CSF and to investigate the regulatory mechanism of MENK on the immmune fuction of DCs, the BM-DCs were prepared and cultured in vitro in the presence of GM-CSF, or MENK alone or in combination of the two factors. The mRNA of TLR-4 was detected by RT-PCR and the protein of TLR-4 was detected by Western-blot technique after seven day＇s culture. Phenotypic features of BM-DCs were analyzed by FACS, the immune function was assayed by means of mixed lymphocyte reaction（MLR）. The results indicate that the expressions of TLR-4 and costimulatory molecules of DC cultured in the midium contained both MENK and GM-CSF were significantly increased compared to those from the normal control group （P〈0.01） and GM-CSF group （P〈0.05）, and these DC had strong ability to trigger MLR These findings suggest that MENK may promote the maturation and expressions of TLR-4 at both mRNA and protein levels, and enhance immunologic functions of BM-DCs.