中文摘要：

为研究牛膝多糖（ABPS）对巨噬细胞的活化作用，以不同浓度的ABPS体外刺激小鼠腹腔来源巨噬细胞，采用ELISA技术检测TNF—α、IL-12的分泌，采用Real．TimePCR检测TLR2／4mRNA的表达。结果显示，浓度为100、200μg／mL的ABPS对巨噬细胞TNF—α分泌均有显著的促进作用；浓度为200μg／mL的ABPS对巨噬细胞IL-12分泌有明显的促进作用。浓度为100、200μg／mL的ABPS能明显上调巨噬细胞TLR4mRNA的表达。这表明，ABPS能激活巨噬细胞。

英文摘要：

The activation effects of ABPS on marcrophagus （Mφ） were investigated. Mφ from abdominal cavity of mice were stimulated in vitro with different concentrations of ABPS, the excretion of TNF-α and IL-12 were tested by enzyme linked immunosorbent assay （ELISA） , and adopted RT-PCR to monitor the effects of ABPS on TLR 2/4 mRNA expression. The results showed that ABPS at concentration of 100 and 200μg/mL remarkably enhanced the excretion of TNF-α. ABPS at the concentration Of 200 μg/mL had significant promotion on IL-12 excretion. And ABPS at the concentration of 100,200 μg/mL could obviously raise Mφ＇s TLR 4 mRNA expression. All these indicated that ABPS can activate Mφ.