中文摘要：

目的：通过体外细胞株研究蛋氨酸脑啡肽（Methionine-enkephalin,MENK）对树突状细胞（Dendritic cell,DC）功能调节的机制,为进一步阐明蛋氨酸脑啡肽的免疫活性提供依据。方法：应用扫描电镜技术、酸性磷酸酶活性检测、流式细胞仪检测技术、酶联免疫吸附试验检测DC表型和功能的各种指标。结果：本实验应用小鼠髓系DC2.4细胞株,电镜观察DC的超微结构,可见10-12mol/L蛋氨酸脑啡肽处理组DC突起增多,形态上更加成熟;DC表面MHCⅡ、CD86和CD40的表达增加;酸性磷酸酶活性降低;DC白细胞介素12（IL-12）的表达增加（P〈0.05）。结论：本实验结果证明了适宜浓度的蛋氨酸脑啡肽促进小鼠髓系DC2.4的表型及功能的成熟。

英文摘要：

Objective：To explore the mechanism of immunomodulatory effects of methionine-enkephalin（MENK）on dendritic cells（DC）.Methods：We used scanning electronic microscope for DC morpholopy,assay for acid phosphatas activity,flow cytometry（FCM） and ELISA to study the effects of DC by MENK.Results：MENK（10-12mol/L） could increase the expression of MHC classⅡ,CD86 and CD40 molecules on DC surface（P0.05）.MENK-treated DCs secreted higher level of IL-12（P0.05）.MENK-treated DC displayed a more matured morpholopy,with long protrusions,while untreated-DCs displayed shorter protrusions.MENK depresses the activity of acid phosphatase in DCs,indicating more maturation.Conclusion：Suitable concentration of MENK can promote the phenotypic and functional maturation of DC markedly.