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Optical detection of acetylcholine esterase based on CdTe quantum dots
  • ISSN号:1001-6538
  • 期刊名称:Chinese Science Bulletin
  • 时间:2013.7
  • 页码:2622-2627
  • 分类:O471.1[理学—半导体物理;理学—物理] Q556[生物学—生物化学]
  • 作者机构:[1]Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beifing 100190, China
  • 相关基金:This work was supported by the National Natural Science Foundation of China (81171454 and 61178035).
  • 相关项目:基于量子点荧光检测酶类肿瘤标志物的生物传感器及其原理研究
中文摘要:

在这研究,我们基于 CdTe 量点(QD ) 为醋胆素 esterase (疼痛) 的察觉构造了一个简单、敏感、快速的生物传感器。由一步舞酶反应的疼痛的察觉限制基于 thiolglycolic 酸(TGA ) 稳定了 QD (TGA-QDs ) 是 10 U/L,线性范围分别地是 10100 和 1001200 U/L。由基于 3-mercaptopropionic 酸(MGA ) ,稳定的 QD (MGA-QDs ) 被发现是 20 U/L 和线性范围的二拍子的圆舞酶反应的疼痛的察觉限制是 1002500 U/L。生物传感器的试验性的条件被优化,并且察觉机制被学习。我们也基于 TGA-QDs 或 MGA-QDs 在浆液样品检测了疼痛。线性范围分别地是 10140 和 501000 U/L。这个新奇生物传感器的优秀表演证明这策略有巨大的潜力在疼痛的实践察觉被使用。

英文摘要:

In this study, we have constructed a simple, sensitive and rapid biosensor for detection of acetylcholine esterase (ACHE) based on CdTe quantum dots (QDs). The detection limit of AChE by one-step enzyme reaction based on the thiolglycolic acid (TGA) stabilized QDs (TGA-QDs) was 10 U/L and the linear range was 10-100 and 100-1200 U/L, respectively. The detection limit of AChE by two-step enzyme reaction based on the 3-mercaptopropionic acid (MGA) stabilized QDs (MGA-QDs) was found to be 20 U/L and the linear range was 100-2500 U/L. The experimental conditions of biosensors were optimized, and the detection mechanism was studied. We also detected ACHE in serum samples based on TGA-QDs or MGA-QDs. The linear range was 10-140 and 50-1000 U/L, respectively. The excellent performance of this novel biosensor demonstrated that this strategy has prodigious potential to be applied in practice detection of ACHE.

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