中文摘要：

活性污泥胞外聚合物（extracellular polymeric substances，EPS）是污水生物处理过程中污泥结构、脱水性能、絮凝性能以及沉降性能的重要决定因素，而提取方法不同EPS的剥离程度存在很大的差别．因此EPS提取方法的建立至关重要．采用6种方法提取不同污泥EPS，分析了不同污泥每g干污泥中分层EPS的质量及组成，并考察了污泥粒径及脱水性能与污泥EPS的关系．结果表明：不同工艺不同污泥每g干污泥中EPS质量不同；甲醛＋NaOH法提取EPS效率高且对细胞破坏程度小，试验中不同污泥EPS提取量为15．88～39．30mg；每g干污泥中黏液层EPS质量越高，蛋白质与多糖质量浓度之比越大，污泥脱水性能越差；污泥EPS的提取效率与污泥粒径存在一定的相关性，污泥粒径越大，提取效率越高．

英文摘要：

Extracellular polymeric substances ( EPS ) is an important determinant of the characters of sludge produced in biological wastewater treatment processes, including the structure, dewaterability, flocculation and settling behavior. However, the different extraction methods of EPS leaded to more discrepancy of EPS stripping degree. Thus, the establishment of the EPS extraction from sludge method is crucial. Six different methods were employed to extract EPS from different sludge in this study, and the content and composition of EPS in different sludge layers were analyzed. Meanwhile, the relationship between the sludge particle size and dewatering performance and the EPS were investigated. Results show that the EPS content is different in different processes of reactor. Formaldehyde + NaOH extraction method was proved to be the most efficient method to extract EPS from sludge, and the destructiveness on cells of this method was relatively slight. The extracted EPS content of different sludge of the method is in the range of 15. 88 -39. 30 mg/gVSS. The content of slime-EPS is higher, the ratio of protein and polysaccharide is larger, but the dewaterability of the sludge is poorer. Besides, the sludge particle size is greater, and the extraction efficiency of EPS is higher.