中文摘要：

为了了解低溶解氧下硝化活性污泥的特性以及相关微生物的种群结构,以人工配水模拟实际生活污水,在SBR反应器中,培养驯化低溶解氧硝化活性污泥,溶解氧浓度为0.3~0.5mg/L,成功驯化后检测其种群数量及结构并对其进行影响因素的分析.结果表明,低氧驯化的硝化活性污泥最适温度为25℃,最适pH值为8.5;在pH值为7.5,温度为20℃,溶解氧在0.5~4mg/L的条件下,比氨氧化速率和比亚硝氧化速率基本保持在0.3和0.6mgN/(mgMLSS.d)左右.通过高通量测序,低氧驯化的硝化反应器中主要亚硝酸氧化微生物为Nitrospira菌属,占总菌属的33%;主要氨氧化微生物为Nitrosomonas菌属,占总菌属的7%.

英文摘要：

The characteristics and microbial community of nitrifying bacteria cultivated at the low DO concentration of0.3~0.5mg/L in a sequencing batch reactor(SBR)was investigated in this study.The bacterial abundance and diversity were analysed with different influencing factors after successful cultivation of the active sludge.Results showed that,the optimum temperature for low-DO nitrifying bacteria was25℃,and the optimum pH was8.5.Under the condition of pH7.5at20℃,specific ammonia oxidate rate and specific nitrite oxidate rate were0.3and0.6mgN/(mgMLSS?d)with DO of0.5~4mg/L,respectively.High throughput sequencing analysis revealed that Nitrospira genus was the dominant nitrite oxidizing bacteria(NOB)accounting for33%of whole community,and Nitrosomonas was the major ammonium oxidizing bacteria(AOB)accounting for7%in the low-DO nitrification reactor.