采用微生物培养法及氯仿熏蒸法分析了退化喀斯特森林恢复过程中不同层次、不同生境、根际和非根际土壤中土壤微生物生物量碳(soil microbial biomass C,SMBC)及微生物熵(qSMBC)的变化特征。结果表明:①退化喀斯特森林恢复过程中SMBC及qsMBC存在较大差异。随着退化喀斯特森林的恢复,SMBC及qSMBC明显上升,表现为乔木群落阶段〉灌木群落阶段〉草本群落阶段〉裸地阶段,反映出土壤质量在逐渐恢复;②SMBC在土壤剖面上均表现明显的垂直变化特征,即随土层深度的增加呈递减趋势,具体表现为A层〉B层,但qSMBC随土层深度的增加呈递增趋势;③SMBC的变化特征在不同生境间表现为:除裸地恢复阶段外,其它三个阶段总体上表现为石沟比石槽和土面两个小生境偏大的特点,这是因为石沟生境有利于微生物类群和土壤动植物群体的繁殖,但qSMBC的变化刚好与之相反;④SMBC及qSMBC根际和根外变化明显,前者表现为根际〉非根你,后者表现为根际〈非根际。
The changes of SMBC and qSMBC of different soil layer, different microhabitats and rhizosphere, non-rhizosphere were studied during the process of degraded karst forest restoration by using the methods of microbial incubation and the chloroform fumigation-direct extraction. The results showed that SMBC and qSMBC had obvious difference. With degraded karst forest restoration, SMBC and qSMBC were ascending obviously, taking on arboreal community stage 〉shrubby community stage 〉herbaceous community stage 〉bare land stage, which indicated that the soil quality has been restored gradually. The SMBC and qSMBC took on the characteristic of vertical change in the soil profile, which decreased with the increase of the soil depth, presenting A 〉B. But change of qSMBC was opposite, it decreased with the forest restoration. The changes of SMBC in different habitats overall performed the characteristics which stone gully was higher than soil surface and stone-groove, It was because stone gully habitat were advantageous to breed soil microorganisms and animal and plant groups. But change of qSMBC was just opposite. The SMBC and qSMBC were different between the rhizosphere and none-rhizosphere. The former performed obviously rhizosphere 〉 non-rhizosphere, the later performed rhizosphere〈non-rhizosphere.