中文摘要：

采集不同恢复阶段的土壤样品，采用微生物常规分析法测定土壤中氨化细菌、硝化细菌、纤维素分解菌及固氮菌的数量，研究退化喀斯特植被恢复过程中不同生境、不同层次及根际和非根际土壤微生物生理类群数量分布特征。试验结果表明：随着退化喀斯特植被的恢复，土壤氨化细菌、硝化细菌、纤维素分解菌及固氮菌的数量明显上升，表现为乔木群落阶段〉灌木群落阶段〉草本群落阶段〉裸地阶段（P〈0．01）；土壤剖面上土壤微生物数量均呈明显的垂直分布特征，即随土层深度的增加，呈递减趋势，具体表现为A层〉B层（P〈0．05）。土壤微生物生理类群数量的分布特征在不同生境间表现为：除裸地恢复阶段外，其它三个阶段总体上表现为石沟比石槽和土面两个小生境偏大的特点，但各恢复阶段不同生境间差异不显著（P〉0．05）；根际和根外变化明显，表现为根际〉非根际（P〈0．05）。退化喀斯特植被恢复增加了土壤微生物生理类群数量，使土壤质量得以提高。

英文摘要：

The soil samples were collected of different restoration stage. The amount distribution of soil microbial physiological group with different microhabitats, different soil layer, rhizosphere and non-rhizosphere were studied during degraded Karst vegetation restoration by using the microbial incubation methods. The experimental results showed that the amount of ammonifiers, nitrifying bacteria, cellulose decomposing microbes, nitrogen-fixing bacteria were ascending obviously with degraded Karst vegetation restoration, taking on arboreal community stage 〉 shrubby community stage 〉herbaceous community stage 〉bare land stage （p〈0.01）. It took on the characteristic of vertical distribution in the soil profile, which decreased with the increase of the soil depth, presenting A〉B（p〈0.05）; The amount distribution of soil microbial physiological group in different habitats overall performed the characteristics which stone gully was higher than soil surface and stone-groove, The difference of different habitats in each recovery stage was not significant （P 〉0.05）. The soil amounts of microbial physiological group were different between the rhizosphere and non-rhizosphere. The former was higher than the later （p〈0.05）. The soil amounts of microbial physiological group increased with vegetation restoration of degraded Karst, which indicated the soil quality has been improved gradually.