中文摘要：

目的：研究微流控芯片精子优选技术对精子常规参数及DNA完整性的影响。方法：自行设计制作微流控芯片，利用芯片处理技术和上游法对40例精液标本进行精子优选，通过计算机辅助精液分析系统及染色质扩散试验从精子常规参数及DNA完整性两个方面评价精液体外处理对精子的影响。结果：精液经微流控芯片法和上游法处理后，精子活力、精子正常形态率以及精子尾部肿胀率均有显著提高（P〈0．01），精子的DNA损伤率明显降低（P〈0．01）。微流控芯片法与上游法相比，优选后前者精子DNA损伤率明显低于后者[（8．4±5．8）％vs（16．4±9．2）％，P〈0．01]，而其他参数差异无显著性。结论：微流控芯片技术在精子优选中能获得精子DNA损伤程度小的高质量精子。

英文摘要：

Objective： To investigate the effects of a microfluidic sperm sorter on the routine parameters and DNA integrity of human sperm. Methods： We divided 40 semen samples into two aliquots and performed sperm sorting using a self-made polydimethylsiloxane microfluidic sperm sorter and the swim-up method, respectively. Then we evaluated and compared the effects of these two methods on the sperm routine parameters and DNA integrity by computer-assisted sperm analysis and sperm chromatin disper- sion test. Results ： After processing, sperm motility, normal morphology and tail hypoosmotic swelling rate were significantly improved, while sperm DNA damage remarkably decreased （ P 〈 0.01 ）. The mierofluidic sperm sorter achieved a significantly lower rate of sperm DNA damage than the swim-up method （ [ 8.4 ± 5.8 ] % vs [ 16.4 ±9.2 ] %, P 〈 0.01 ）, but no statistically significant differences were found in all other parameters between the two methods. Conclusion： High-quality sperm with less DNA integrity damage could be obtained in sperm sorting with the microfluidic sperm sorter.