中文摘要：

以锈叶杜鹃无菌种子萌发后的胚轴和子叶为试材,对影响其愈伤组织诱导和增殖的几个关键因子进行了研究。结果表明：MS培养基较适宜愈伤组织的诱导和培养;TDZ和NAA浓度对愈伤组织诱导有很大影响,TDZ 0.50mg/L＋NAA 0.05mg/L对愈伤组织诱导效果较好,诱导率为77.78%;MS＋TDZ 0.10mg/L＋NAA 0.05mg/L对愈伤组织增殖效果较好,增殖率为96.67%,增殖倍数为4.50;愈伤组织需及时转瓶,转瓶时间较晚容易褐化死亡,转瓶时间以40d为宜。

英文摘要：

Taking cotyledon and hypocotyl of seed of Rhododendron siderophyllum as experiment material, the several key factors on callus induction and multiplication of Rhododendron siderophyllum in tussue culture were studied. The results showed that MS medium was suitable for induction and multiplication of callus. The concentrations of TDZ and NAA had a strong influence on callus induction. The MS medium supplemented with TDZ 0. 50 mg/L and NAA 0. 05 mg/L had high induction effect,and the induction rate was 77. 78%. The MS medium supplemented with TDZ 0. 10 mg/L and NAA 0. 05 mg/L was suitable for callus multiplication,the multiplication rate could reach 96. 67% ,the multiplication ratio was 4. 50. In order to keep callus multiplication more and more, the callus needed to transfer medium again and again. The ideal time for transferring medium was 40 days, so that it could avoid browning of callus.