中文摘要：

为了建立绿竹高效愈伤组织诱导体系,研究了小穗的生物学特性,以及不同激素种类及浓度组合、单独添加2,4-D（2,4-Dichlorophenoxyacetic acid）浓度、有机添加物、甘露醇预处理等对小穗愈伤组织诱导的影响。结果表明：0.8～1 cm的小穗是诱导愈伤组织的最适宜材料;2,4-D与NAA组合小穗的愈伤组织诱导率最高,愈伤组织的质量也较好;小穗愈伤组织诱导以MS基本培养基添加3 mg·L^-12,4-D、1 mg·L^-1NAA和500 mg·L^-1Proline、500 mg·L^-1Glutamine、300 mg·L^-1Casein Hydrolysate较好,愈伤组织诱导率最高,质量也相对较好;甘露醇预处理小穗愈伤组织出现时间推迟,愈伤组织质量与未处理的没有显著差异。

英文摘要：

To establish efficient system of callus induction, floral biology of Bambusa oldhami was studied. Meanwhile, the effects of external factors such as the combinations of different phytohormones, the concentrations of 2,4-Dichlorophenoxyacetic acid （2,4-D）, the supplement of organic additives and the mannitol pretreatment on callus induction of spikelets were tested. The results showed that the spikelets with 0.8 - 1 cm were the most appropriate for callus induction. The highest induction rate was achieved on MS medium supplemented with 2,4-D and NAA. Further, the basic MS medium added with 3 mg·L-1 2,4-D, 1 mg·L-1 NAA, 500 mg·L-1 Proline, 500 mg·L-1 Glutamine and 300 mg·L-1 Casein Hydrolysate was the best induction medium. The callus induction was delayed with the mannitol pretreatment, while the quality of callus was not improved.