为更好地开展竹类植物的杂交育种工作,对雷竹花粉的萌发率及贮藏方法进行了研究。结果表明,利用离体花粉萌发法测定的雷竹花粉萌发率远低于氯化三苯四氮唑(TTC)染色法测定的花粉生活力;添加50mg·L^-1硼酸有利于雷竹花粉萌发;不同发育阶段的花粉萌发率有明显差异,以花药完全露出未开裂的花粉萌发率为最高;花粉采集时间以15.30优于9:30;雷竹花粉不耐贮藏,4℃湿藏有利于保持花粉生活力。
Pollen germination and storage of Phyllostachys violascens were studied for bamboo crossbreeding. The results were sum- marized as follows: the pollen germination rate measured by in vitro pollen germination was lower than the pollen viability rate detec- ted by triphenyltetrazolium chloride triphenyhetrazolium chloride (TIC) staining technique; the pollen germination ability could be improved by supplementing with 50 rag^-1 boric acid in the medium; the pollen germination rate of the anthers completely outcrop but not dehiscent was higher than those of other development stages ; as for the pollen collection time, 15:30 was better than 9:30 ; pollen viability of Ph. violascens would be lost quickly during storage, and certain humidity and low temperature at 4℃ could be ben- eficial to keep the pollen viability.