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雷竹花粉萌发率及贮藏方法研究
  • ISSN号:1001-389X
  • 期刊名称:福建林学院学报
  • 时间:2012.4.4
  • 页码:146-150
  • 分类:S795.9[农业科学—林木遗传育种;农业科学—林学]
  • 作者机构:[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300
  • 相关基金:国家自然科学基金资助项目(31000295);浙江省优先主题重点农业项目(2010C12011).
  • 相关项目:竹子成花逆转体系的建立
中文摘要:

为更好地开展竹类植物的杂交育种工作,对雷竹花粉的萌发率及贮藏方法进行了研究。结果表明,利用离体花粉萌发法测定的雷竹花粉萌发率远低于氯化三苯四氮唑(TTC)染色法测定的花粉生活力;添加50mg·L^-1硼酸有利于雷竹花粉萌发;不同发育阶段的花粉萌发率有明显差异,以花药完全露出未开裂的花粉萌发率为最高;花粉采集时间以15.30优于9:30;雷竹花粉不耐贮藏,4℃湿藏有利于保持花粉生活力。

英文摘要:

Pollen germination and storage of Phyllostachys violascens were studied for bamboo crossbreeding. The results were sum- marized as follows: the pollen germination rate measured by in vitro pollen germination was lower than the pollen viability rate detec- ted by triphenyltetrazolium chloride triphenyhetrazolium chloride (TIC) staining technique; the pollen germination ability could be improved by supplementing with 50 rag^-1 boric acid in the medium; the pollen germination rate of the anthers completely outcrop but not dehiscent was higher than those of other development stages ; as for the pollen collection time, 15:30 was better than 9:30 ; pollen viability of Ph. violascens would be lost quickly during storage, and certain humidity and low temperature at 4℃ could be ben- eficial to keep the pollen viability.

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