中文摘要：

为研究小花碱茅（Puainellia tenuiflora）K^＋/Na^＋选择吸收分子机制,根据已报道的其他植物AKT1基因的保守序列设计一对简并性引物,以小花碱茅根部总RNA为模板,采用RT-PCR方法克隆出AKT1基因,以期为小花碱茅AKT1全长基因的克隆、表达调控及其作物改良的研究奠定基础。序列分析结果表明：该基因长度大约为1019 bp,编码339个氨基酸;所得序列与GenBank中注册的高等植物AKT1基因序列的同源性均在85%以上,与其他AKT1的氨基酸序列同源性达73%以上。

英文摘要：

Degenerate primers were designed based on the conserved sequences of the AKT1 genes from other plants for investigating molecular mechanisms of K^＋/Na^＋ selective absorption in P.tenuiflora.Total RNA was extracted from the roots of Puccinellia tenuiflora to obtain an AKT1 gene fragment by reverse transcription polymerase chain reaction（RT-PCR）.The sequencing result shows that the AKT1 gene fragment（about 1019 bp） from Puccinellia tenuiflora encodes 339 amino acids.Homology comparison show that the AKT1 gene fragment contains over 85% nucleotide and 73% amino acid homology when compared with other plants in the GenBank having an AKT1 gene.These results offer further understanding for full-length AKT1 gene cloning,gene expression regulation and crop improvement.