欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
位置：立项数据库 > 立项详情页
中文摘要：
本项目取得了以下主要进展和成果（1）发现干旱胁迫下荒漠旱生植物霸王叶中Na+浓度显著增加，Na+对叶渗透势的贡献由8%增至13%；50 mM NaCl处理使干旱胁迫下叶中Na+浓度增加了2.3倍，Na+对叶渗透势的贡献增至28%；尽管干旱胁迫下霸王体内Na+浓度大幅度增加，但其根和叶中的K+浓度仍然能够维持稳定。表明干旱胁迫下Na+对叶渗透势贡献的增加及维持其K+浓度的稳定是霸王适应干旱生境的重要原因。（2）从霸王中鉴定了离子区域化功能基因ZxNHX和ZxVP1；发现在盐（5-150 mM NaCl）或干旱（50%-15% FWC）处理下，霸王叶中ZxNHX的表达与Na+积累间呈显著正相关；25-100 mM NaCl下ZxNHX抑制剂amiloride处理破坏了细胞的离子区域化能力，使霸王叶中Na+浓度显著下降了24%-38%，明显消弱了植株的抗逆能力。（3）首次获得了超表达ZxVP1-1及ZxNHX-ZxVP1-1的百脉根强耐盐抗旱株系；发现后者比前者表现更为优越，具有更强的抗逆能力。（4）建立了霸王的高频组培再生体系，为今后采用RNAi技术研究其抗旱功能基因奠定了坚实的基础。
结论摘要：
英文主题词Na+ compartmentalization; Succulent xerophytes;tonoplast Na+/H+ antiporter; H+-PPase; Drought- and salt resistance
成果综合统计
期刊论文
会议论文
专利
获奖
著作
期刊论文
The ZxNHX gene encoding tonoplast Na+/H+ antiporter in the xerophyte Zygophyllum xanthoxylum plays i
Amiloride reduces sodium transport and accumulation in the succulent xerophyte Zygophyllum xanthoxyl
Overexpression of the Arabidopsis H+-PPase enhanced resistance to salt and drought stress in transge
会议论文
Molecular cloning a tonoplast proton pyrophosphatase gene (ZxVP1-1) from Zygophyllum xanthoxylum and
The ZxNHX gene encoding vacuolar Na+/H+ antiporter in the xerophyte Zygophyllum xanthoxylum plays im
The establishment of a regeneration system of Lotus corniculatus L. and its genetic transformation s
Overexpression of the Arabidopsis H+-PPase gene enhanced salt and drought tolerance of transgenic Lo
solation and functional characterization of vacuolar Na+/H+ antiporter gene (ZxNHX) from the succule
Molecular cloning and expression analysis of a potassium channel gene (ZxAKT1) from the succulent xe
Overexpression of the Arabidopsis AVP1 gene enhanced the salt- and drought-tolerance in transgenic a
Puccinellia tenuiflora Retains a Low Na+ Level under Salt Stress by Limiting Unidirectional Na+ Infl
Establishment of a high-frequency regeneration system of succulent xerophyte Zygophyllum xanthoxylum
Effects of sodium chloride on the photosynthesis and water status of the succulent xerophyte Zygophy
Molecular cloning and expression of plasma membrane Na+/H+ antiporter gene (SOS1) from the halophyte
Inhibition of vacuolar Na+/H+ antiporter by amiloride reduces sodium accumulation and transport in t
Molecular cloning and characterization of a vacuolar Na+/H+ antiporter gene from the succulent xerop
王锁民的项目