中文摘要：

本研究以多浆旱生植物霸王为材料，通过对不同激素组合和外植体（子叶、茎、茎节、下胚轴）的研究，建立了一个稳定、高效的霸王组培再生体系。结果表明，霸王子叶是诱导愈伤组织的最佳外植体，茎节是诱导不定芽的最适外植体；最佳愈伤组织诱导培养基是含有０．１ｍｇ／Ｌ６-ＢＡ 和１．０ｍｇ／Ｌ ＮＡＡ 的ＭＳ，子叶在此培养基上愈伤组织诱导率为１００％，诱导时间为２０ｄ，优于已报道的３０ｄ；霸王茎节不定芽诱导的最适培养基为附加１．５ ｍｇ／Ｌ６-ＢＡ和０．５ｍｇ／Ｌ ＮＡＡ 的ＭＳ，诱导率高达８２．５％，诱导时间为７周，优于已报道的１０周；诱导生根的最佳培养基为含１．０ｍｇ／Ｌ ＮＡＡ 和０．５ ｍｇ／ＬＩＢＡ 的１／２ ＭＳ，生根率高达８６．７％，比已报道的７３．３％ 的生根率提高了１３．４％，且在此培养基上１３ｄ 开始出现不定根，早于已报道的２０ｄ；本试验从诱导愈伤组织到再生苗成株需（８４±４）ｄ。

英文摘要：

The effect of compounding proportions of different hormones on explants （cotyledon, stem, eustipes, and hypocotyl） were studied in tissue cultures of the succulent xerophyte Zygophyllum xanthoxylum, and a stable, high-efficiency plant regeneration system was established. Cotyledons were the best type of explant for callus induction and eustipes were the best for adventitious bud induction. The best callus inducing medium for Z. xanthoxylum was MS containing 0.1 mg/L 6-BA and 1.0 mg/L NAA, in which the callus induction rate for cotyledons was 100% with an induction time of 20 d, shorter than the previously reported 30 d. The best medium to induce adventitious buds was MS containing 1.5 mg/L 6 BA and 0.5 mg/L NAA, in which shoot differentiation of eustipes reached 82.5% and the induction time wais 7-weeks, shorter than the earlier report of 10- weeks. The best medium for rooting was 1/2 MS containing 1.0 mg/L NAA and 0.5 mg/L IBA, in which the frequency of rooting reached 86. 7%, 13. 4% greater than the previous result of 73. 3%. In this medium, adventitious roots began to appear in 13 d （earlier than the reported 20 d） and the transition from callus to regeneration plant was completed in （84±4） d. regeneration