中文摘要：

从四川省雅安市茶园土壤中分离到1株能高效降解氯氰菊酯的细菌B-1。根据其形态、生理生化特征和16S rDNA序列分析（登录号HQ009796）,将该菌鉴定为Bacillus licheniformis。菌株B-1以共代谢方式降解氯氰菊酯,对20mg/L氯氰菊酯72h降解率达93.93%,对100mg/L氯氰菊酯240h降解率为70.35%,能检测到其降解中间产物3-苯氧基苯甲酸（3-PBA）;菌株B-1对20mg/L溴氰菊酯、氟氯氰菊酯和氰戊菊酯72h降解率分别为95.62%、79.73%和55.67%;菌株B-1对氯氰菊酯降解作用源于其所产生降解酯酶,为非诱导酶,存在于细胞内外,其主要分泌于胞外,同工酯酶电泳进一步表明其胞内外均可能有相同活性降解酯酶带;菌株B-1培养液对小白鼠的急性毒性实验证明其属于实际无毒。

英文摘要：

A cypermethrin-degrading strain named B-1 was isolated from soil samples collected from a tea garden in Ya＇ an, Sichuan province. According to its morphological, physiological and biochemical characteristics and 16S rDNA sequence （GenBank Accession No. HQ009796 ）, B-1 was identified as Bacillus licheniformis. B-1 degraded cypermethrin by a co- metabolic mechanism and the degradation rate was 93.93% for 20 mg/L cypermethrin in 72 h and 70.35% for 100 mg/L cypermethrin in 240 h. 3-PBA was detected as an intermediate product during the degradation process. The degradation rates for deltamethrin, cyfluthrin and fenvalerate at a concentration of 20 mg/L were 95.62%, 79.73% and 55.67% in 72 h, respectively. B-1 could produce a non-inducible esterase that degrades cypermethrin. The enzyme was found both inside and outside the cells mainly as an extracellular product. Isozyme electrophoresis analysis suggested that intracellular and extracellular esterase extracts from strain B-1 had the same esterase electrophoretic pattern. Acute toxicity tests showed that culture supernatant of strain B- 1 had no observed toxicity for mice.