中文摘要：

采用高效液相色谱-紫外（high-performance liquidchromatography with ultraviolet，HPLC—UV）法测定微生物降解体系中3-苯氧基苯甲酸（3-PBA）的含量。以Gemini100A C18柱（150mm×4．60mm，5．0μm）为色谱柱，乙腈-水（70：30，V／y）为流动相，流速0．7mL／min，用紫外检测器在210nm处检测3-PBA。结果显示：3-PBA对照品的保留时间为4．268min，线性范围为0．5～50．0mg／L，3-PBA平均回收率为98．919％，RSD为2．78％；运用该方法测得4种不同来源的混合菌株发酵液中3-PBA的残留量分别为24．467、86．266、2．633、1．921mg／L。本法简单、快速、准确、分离度好。

英文摘要：

A HPLC method with UV detection was proposed to determine the concentration of 3-phenoxybenzoic acid （3-PBA） in microbial degradation systems. The chromatographic separation was performed on a Gemini 100A C18 column （5.0 μm, 150 mm× 4.60 mm, i.d.） using a mobile phase made up of acetonitrile and water （70：30, V/V） at a flow rate of 0.7 mL/min, and 3- PBA was detected at 210 nm using a UV detector. The retention time of 3-PBA was 4.268 min. The linear range of the method was 0.5--50.0 mg/L. The average spike recovery for 3-PBA was 98.919%, with a relative standard deviation （RSD） of 2.78%. The residues of 3-PBA in 4 different microbial degradation systems were 24.467, 86.266, 2.633 mg/L and 1.921 mg/L, respectively. The developed method demonstrated the benefits of simplicity, rapidity, high accuracy and good separation.