以对氯氰菊酯农药具有较强耐受性和降解率的酿酒酵母菌作为研究对象,考察不同因素对该菌株降解氯氰菊酯特性的影响,进而探讨酿酒酵母生长量与氯氰菊酯降解率间的内在联系。结果显示,不同因素对酿酒酵母降解氯氰菊酯具有显著影响;当培养基中氯氰菊酯浓度为200mg/L,初始pH为7.0,250mL锥形瓶装液量为20mL,接种量为5%(v/v,菌体浓度为3.0×10^7个/mL),摇床转速为140r/min,培养温度为31℃,培养时间为96h时,在此条件下,氯氰菊酯的降解率可达38.52%。
The objective of this research was to investigate the effect of environmental factors on the degradation characteristics of cypermethrin by Saccharomyces cerevisiae with high tolerance and degradation rate on cypermethrin,and to discuss the internal relations between degradation rate of cypermethrin and biomass of the strain.The results indicated that environmental factors had a significant effect on the degradation of cypermethrin by Saccharomyces cerevisiae.Meanwhile,the optimum conditions for degradation of cypermethrin were as follows: cypermethrin 200mg.L-1,initial pH 7.0,substrate 20mL,inoculum 5%(v/v,3.0×107cells/mL),rotation speed 140 r.min-1,incubation temperature 31°C and incubation period 96h,then the degradation rate of cypermethrin reached 38.52%.