中文摘要：

目的探讨LW-AFC对免疫功能低下小鼠是否有改善作用。方法昆明小鼠ip给予环磷酰胺(CTX)0.02g·kg-1,连续10d,制备免疫功能低下小鼠模型。六味地黄(LW)浓缩丸(1.4g·kg-1)和LW-AFC(0.2,0.4和0.8g·kg-1)组小鼠在每次给予CTX后ig给予相应药物,连续10d。监测小鼠体重,以及胸腺和脾脏重量变化,并计算胸腺和脾脏系数;[3H]TdR掺入法检测脾淋巴细胞增殖反应,MTT法检测脾脏自然杀伤(NK)细胞杀伤活性,流式细胞术检测脾淋巴细胞中CD4+CD8-和CD4-CD8+T细胞亚群百分率,并计算CD4+/CD8+T细胞亚群比值。结果与正常对照组比较,模型组小鼠胸腺和脾重量及其系数明显降低,体重增长显著缓慢;LW和LW-AFC对小鼠体重增长缓慢、胸腺重量及其系数降低无明显改善作用;LW-AFC0.2g·kg-1对CTX导致的脾重及系数降低有明显改善(P<0.05)。模型组小鼠脾淋巴细胞自发增殖反应、刀豆蛋白A(ConA)和脂多糖(LPS)诱导的脾细胞增殖反应与正常对照组比较均明显降低,[3H]TdR掺入值由正常对照组的6115±441,19432±1778和(23345±72...

英文摘要：

OBJECTIVE To investigate the improvement of LW-AFC on immunodeficiency. METHODS An immunodeficiency model of Kunming mice was established after mice were ip given CTX 0. 02 g·kg-1,once a day,for 10 d. Liuwei Dihuang concentrated pills ( LW) 1. 4 g·kg-1 and LW-AFC 0. 2,0. 4 and 0. 8 g·kg-1 were ig given in LW and LW-AFC groups,respectively, followed by daily administration of CTX for 10 d. The body weight of mice was measured,once every 2 d. On the 11th day the mice were sacrificed,the thymus and spleen were...