中文摘要：

目的比较六味地黄汤全方（LW）及其拆方三补（地黄、山茱萸、山药）和三泻（茯苓、泽泻、牡丹皮）对快速老化小鼠（SAM）免疫功能的调节作用。方法分别灌胃给予SAM亚系小鼠（SAMP8）LW（10g/kg）、三补（6.4g/kg）和三泻（3.6g/kg）,每日1次,连续60d;抗快速老化亚系小鼠（SAMR1）作为对照。采用3H-TdR掺入法检测脾脏淋巴细胞增殖能力,流式细胞术观察脾脏CD3＋、CD4＋、CD8＋、CD19＋淋巴细胞百分率。结果与SAMR1组相比,SAMP8组经刀豆蛋白A（ConA）和脂多糖（LPS）诱导的脾细胞增殖能力、脾脏CD3＋、CD4＋细胞百分率、CD3＋/CD19＋和CD4＋/CD8＋比值均显著下降,而CD19＋细胞百分率显著上升;灌胃给予LW全方及折方对上述指标具有不同程度的改善作用,其中,LW对LPS诱导的脾细胞增殖能力、CD3＋和CD19＋细胞百分率、CD3＋/CD19＋比值、CD4＋细胞百分率及CD4＋/CD8＋比值的改善作用优于三补和三泻;三补对升高ConA诱导的脾细胞增殖能力优于LW和三泻,对脾脏CD3＋和CD19＋细胞百分率、CD3＋/CD19＋细胞比值及CD4＋细胞百分率的改善作用优于三泻;三泻升高CD4＋/CD8＋比值的作用优于三补。结论 LW可显著改善SAMP8低下的T、B淋巴细胞功能,纠正脾脏CD4＋/CD8＋T细胞亚群比例失衡,其作用优于单独应用三补和三泻;三补和三泻对SAMP8的免疫改善作用各有侧重,三补的作用可能在于调节T、B淋巴细胞的数量和功能,三泻则可能着重于调节T细胞亚群的比例。本研究提示LW对免疫功能的调节是三补和三泻相互协调综合作用的结果 ,该结果为揭示LW配伍规律及其科学内涵提供了一定的实验依据。

英文摘要：

Objective To investigate the effects of Liuwei Dihuang decoction （LW）,its San-bu （three tonics） and San-xie （three eliminators） ingredients on regulating the immune function of senescence-accelerated mice（SAM）. Methods SAM-prone（SAMP）8 mice were orally administrated with LW （10 g/kg）,San-bu （6.4 g/kg） and San-xie （3.6 g/kg） respectively,once per day for 60 days,while SAM-resistant（SAMR）1 mice administrated with distilled water as control group. 3H-TdR incorporation was applied for detecting splenocyte proliferation. Flow cytometry（FCM）was used to observe the percentage of CD3＋,CD4＋,CD8＋ and CD19＋ lymphocytes in the spleens. Results Compared with SAMR1 group,the splenocyte proliferation induced by ConA an LPS,percentages of CD3＋,CD4＋,CD8＋ and CD19＋ lymphocytes and ratios of CD3＋/CD19＋ and CD4＋/CD8＋ in spleens decreased,while percentage of CD19＋ lymphocytes increased in SAMP8 group. After administration of LW,San-bu and San-xie,the above indexes were improved to various degrees. LW showed better effects on improving LPS-induced splenocyte proliferation,percentages of CD3＋,CD19＋ and CD4＋ lymphocytes,and the ratios of CD3＋/CD19＋ and CD4＋/CD8＋ than San-bu and San-xie. San-bu showed better effect on improving ConA-induced splenocyte proliferation than LW and San-xie,and better effect on improving the percentages of CD3＋,CD19＋ and CD4＋ lymphocytes in the spleens and the ratios of CD3＋/CD19＋ than San-xie,while San-xie had better effect on regulating CD4＋/CD8＋ ratio than San-bu. Conclusion These results suggest that LW regulates the function of T and B lymphocytes and the imbalance of subsets of CD4＋ and CD8＋ lymphocytes in the spleens of SAMP8. LW shows better effects than San-bu or San-xie used alone,while San-bu and San-xie have their particular effects. The effects of LW on regulating the immune function might be the integral results of San-bu and San-xie. The results provid some experimental evidences for revealing the c