中文摘要：

目的：基于UPLC-代谢组学技术和预防性给样法研究有氧运动结合银杏叶提取物对高脂血的改善作用。方法：用高脂饲料喂饲SD大鼠4周，同时进行4周的银杏叶提取物（BGE）、有氧运动、BGE结合有氧运动结合干预。实验第1天和第2周末眼眶静脉丛取血，第4周后处死动物，采用基于UPLC-Q／TOF／MS代谢组学方法证明不同治疗组的治疗效果。比较血清TC、TG、HDL-C和LDL-C含量，同时测定血清SOD、GSH—Px、CAT和MDA的浓度。结果：代谢组学方法与生化指标的结果结合证明在4周时间，高血脂症模型被成功建立，不同的时间段产生不同的损伤程度且明确了高血脂症模型的时间进展轨迹，显示了不同治疗组的对高脂血症的影响。结果证明BGE对高血脂症有防治作用，尤其是BGE与有氧运动结合的作用好于仅用BGE或有氧运动的效果。生化指标显示BGE和有氧运动均可显著降低大鼠血清TC、TG和LDL-C提高；BGE提取物结合有氧运动组大鼠血清SOD、GSH—Px、CAT显著升高，同时MDA的水平下降。结论：BGE提取物结合有氧运动可治疗高血脂症大鼠血脂异常。代谢组学可以作为一种推测药理模型制作程度及是否成功的新方法，代谢组学也作为药物或有氧运动改善疾病程度及效果的一个新方法。

英文摘要：

This study was to observe the ameliorative effects of UPLC-based metabonomics and prevention therapy through extraction of Ginkgo biloba （GBE） in combination with aerobic exercise in hyperlipidemia rat. The rats were raised by high fat diet for four weeks. Meanwhile, they were treated with GBE and aerobic exercise for four weeks. The blood sam- ples were collected from retro-orbital puncture with ether and transferred into tubes on the first day and the second week- end. Then those rats were terminated, and the model of hypenlipemia was established and verified by UPLC-Q/TOF/MS method. TC, TG, LDL-C and HDL-C of the serum were detected and the concentration of SOD, GSH-Px, CAT and MDA were measured. According to metabonomic method and biochemical parameters, the hyperlipemia rat model was successfully established in four weeks. The results showed the progress of metabolic disorders in the different periods. The results showed that GBE with aerobic exercise could decrease remarkably the concentrations of LDL-C, TC and TG. The activity of GSH-Px, SOD and CAT in GBE with aerobic exercise group was promoted and the level of MAD decreased dramatically. It could be concluded that GBE in combination with aerobic exercise could treat the abnormity of serum lipid and had obvious anti-lipoperoxidation effect. The model of hypenlipemia was established. The metabolomics technology could be used as a new method whether successful pharmacology model was established or not. Metabolomics could also be a new method for studying on the therapeutic effect of aerobic exer- else.