中文摘要：

利用二乙胺基乙基琼脂糖凝胶柱层析纯化自养小球藻（Chlorella autotropica）粗多糖，得到3个硫酸多糖组分（A，B，C）；利用高效液相色谱仪和熔点仪测定了3个多糖的分子质量和熔点；利用BaCl2浊度法测定了硫酸多糖中硫酸根的质量分数；利用傅立叶变换红外光谱分析了海水自养小球藻3个硫酸多糖组分的环状结构、半缩醛羟基构型及取代基类型。结果显示，3个硫酸多糖组分的硫酸根的质量分数分别为0．77％，8．46％，2．69％，3个组分均含有硫酸基、酰氨基，葡萄糖是3个硫酸多糖组分的主要组成单糖；3个硫酸多糖组分组成单糖的环构型均是吡喃环，成苷的半缩醛羟基构型有一定差异，A是α构型，B、C是α、β两种半缩醛羟基构型并存。为深入研究自养小球藻硫酸多糖的结构及抗癌活性提供基础。

英文摘要：

In order to research the structure and anti-HIV activity of sulfate polysaccharides, three white refined sulfate polysaccharides （A, B, C） were gained with the DEAE sepharose column chromatographic analysis; molecular weights and melting points of three sulfate polysaccharides were got with the HPLC technology and melting point apparatus; determination of sulphate content was made by barium chloride gravimetric method; the chemical compositions and configuration of marine Chlorella autotropica polysaccharides basicly extracted from marine Chlorella autotropica were studied by FT-IR spectroscopy. The results show that sulphate contents of three sulfate polysaccharides were 0.77%, 8.46%, 2.69% respectively, all the three marine Chlorella autotropica polysaccharides have acylamino radical and sulfate radical. Glucose is always a dominant monosaccharide in the three sulfate polysaccharides. Pyranose is the major component of the sulfate polysaccharide in the A, with one glycoside configuration, α-hemiacetal hydroxyl. Pyranose is also the major component of the sulfate polysaccharide in the B and C, with two glycoside configurations, α-hemiacetal hydroxyl and β-hemiacetal hydroxyl coexisted.