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中文摘要：
本试验旨在研究在培养液中添加不同浓度的NO供体硝普钠（sodium nitro-prusside,SNP）（0、0.001、0.01、0.1、1.0 mmol/L）处理,研究培养4 d后的腔前卵泡颗粒细胞凋亡情况。结果表明,1.0 mmol/L SNP组凋亡率显著高于其他各组（P〈0.05）,而0.001 mmol/L SNP组凋亡率显著低于其他各组（P〈0.05）。说明高浓度的SNP对猪腔前卵泡颗粒细胞凋亡起促进作用,低浓度的SNP起抑制作用。
英文摘要：
In order to understand the effects of nitric oxide（NO） on granulose apoptosis of porcine preantral follicles at four days of the culture,we treated the follicles with different concentrations of sodium nitro-prusside（0,0.001,0.01,0.1 and 1.0 mmol/L SNP）,a nitric oxide donor.The results indicated that 1.0 mmol/L SNP was significantly higher than other treatments（P0.05）,0.001 mmol/L SNP was significantly lower than other treatments（P0.05）.In conclusion,high concentrations of SNP in culture medium can significantly stimulate follicles granulose apoptosis,low concentrations of SNP inhibit follicles granulose apoptosis.
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