中文摘要：

旨在研究在培养液中添加不同浓度（0、0.001、0.01、0.1和1 mmol.L^-1）的一氧化氮供体硝普钠（Sodium Nitroprusside,SNP）对猪腔前卵泡体外生长发育的影响。结果显示,体外培养后,各处理组卵泡直径均增加,但未达显著水平（P〉0.05）;第6天1 mmol.L^-1SNP处理组卵泡存活率要显著低于1μmol.L^-1SNP（61.61%vs81.52%,P〈0.05）,与其它各组间差异不显著（P〉0.05）;卵泡成腔率在第4天以1μmol.L^-1SNP组最高,达到50%;第6天,1μmol.L^-1SNP组的卵泡成腔率显著高于0.1和1 mmol.L^-1SNP组（73.07%vs50%,47.62%,P〈0.05）,也高于对照组和0.01 mmol.L^-1SNP组,但差异不显著（P〉0.05）。培养结束（6 d）后,除1mmol.L^-1SNP组卵母细胞正常率显著低于1μmol.L^-1SNP组（71.21%vs 48.18%,P〈0.05）外,其余各组间差异不显著（P〉0.05）,其中0.001 mmol.L^-1SNP组卵母细胞正常率最高,为71.21%;0.001 mmol.L^-1SNP组COC回收率要显著高于其余各组（37.27%vs 22.88%、25.59%、20.74%和19.39%,P〈0.05）。结果表明,NO对猪腔前卵泡的存活、发育成腔及卵母细胞发育都有促进作用,但高浓度会产生毒性作用。

英文摘要：

In order to understand the effects of nitric oxide（NO） on the growth and development of porcine preantral follicle,we treated the follicles with different concentrations of sodium nitroprusside（SNP,0,0.001,0.01,0.1 and 1 mmol·L-1）,a nitric oxide donor.The results showed that the follicle diameter increased during in vitro culture,but there were no significant differences（P〉0.05）;the survival rate in 1 mmol·L-1 SNP group was significantly lower than that in 1 μmol·L-1 SNP group（61.61% vs 81.52%,P〈0.05）,but no significant differences were found between other treatments（P〉0.05）;the antrum formation rate in 1 μmol·L-1 SNP group reached highest to 50% on day 4,and the rate in 1 μmol·L-1 SNP group on day 6 was higher than that of 0.01 mmol·L-1 SNP;In addition,the antrum formation rate in 1 μmol·L-1 SNP group was significantly higher than that in 0.1 and 1 mmol·L-1 SNP group（73.07% vs 50%,47.62%,P〈0.05）.After 6 days′culutre,the oocyte normal rate in 1 mmol·L-1 SNP group was significantly lower than that in 1 μmol·L-1 SNP group（71.21% vs 48.18%,P〈0.05）,and there were no significant difference between other treatments（P〉0.05）.The recovery rate of cumulus cells oocyte complexes（COCs） in 1 μmol·L-1 SNP group was significantly higher than that of control and any other treatments（37.27% vs 22.88%,25.59%,20.74% and 19.39%,P〈0.05）.The results indicated that during the in vitro culture of porcine preantral follicle,low concentration of NO which is released from SNP improves the oocyte growth,development and follicle antrum formation while high level of NO is toxic to follicle survival.