中文摘要：

调查规模化鸡场中产CMY-2大肠杆菌耐药基因与毒力基因的流行现状及共转移情况，对549株来源于鸡场粪样和苍蝇的大肠杆菌进行bla CMY-2 PCR检测；K—B法检测耐药表型；PCR扩增19种相关耐药基因和14种毒力基因；接合试验和质粒复制子分型研究耐药基因与毒力基因的共转移．结果显示：6．0％（33／549）大肠杆菌呈CMY-2阳性，且均为多重耐药；检测到15种耐药基因和4种毒力基因，其中floR，bla TEM-1，sul2，sul1，traT．VagC检出率较高；63．6％产CMY-2大肠杆菌中bla CMY-2基因可通过lncA／C或lncI1质粒与耐药基因和（或）毒力基因共转移．本研究表明规模化鸡场粪样和苍蝇源产CMY-2大肠杆菌已成为耐药基因和毒力基因的重要储存库，携带二者质粒的转移导致耐药与毒力的传播扩散．

英文摘要：

The co-transfer of resistance genes and virulence genes among CMY-2-producing- Escherichia coli from large-scale poultry farms in China was investigated. PCR was used to examine the presence of bla CMY-2 in 549 E. coli. Antimicrobial suseeptibilities were tested by diffusion methods. Gene detection and co-transfer were performed for 19 resistance genes and 4 virulence genes by PCR, conjugation experiment, and PBRT. Results showed 6. 0 %（33/549）isolates with multiple resistance carried bla CMY-2 gene. 15 resistance genes and 4 virulence genes were detected with high rate of floR, bla TEM-1, sul2, sul1, traT, and VagC. Co-transfer of resistance genes and virulence genes byplasmidsoflncA/Cor lncI1 was detected in 63.6 % CMY-2-positive-E. coli. This study demonstrated E. coli producing CMY-2 has become reservoirs of resistance genes and virulence genes and plasmids coharhoring these two factors lead to its dissemination among large-scale poultry farms.