中文摘要：

目的观察不同碘营养水平下大鼠妊娠期碘代谢水平的变化情况。方法雌性Wistar大鼠150只，体质量80-100g，按体质量分层后随机分为5组：对照组（NI），低碘1、2组（L11、L12），高碘1、2组（H11、H12），每组30只。分别饮用含碘离子（I^-）50μg／L（NI）、0wg／L（L11）、5μg／L（1212）、3000μg／L（H11）、10000仙以（H12）的去离子水。饲养12周后收集雌鼠尿液，测定基础尿碘值，然后将雌鼠同雄鼠合笼交配。分别于孕早期（第6—7天），孕中期（第12～13天）、孕晚期（第19～20天）处死，收集血清、羊水。采用砷铈催化分光光度法测尿碘及羊水碘，温和酸消化法测血清碘。结果基础尿碘中位数L11组、L12组（5．96、15．92μg／L）均低于NI组（43．75μg／L，P均〈0．01）；H11组、H12组（5263．96、20389．64μg／L）均高于NI组（P均〈0．01）。各组孕期尿碘中位数低于基础尿碘中位数（P均〈0．01）。NI组孕早、中期尿碘中位数（28．97、34．34μs／L）低于孕晚期（42．31μg／L，P均〈0．01）。血清碘L11组、L12组[（3．68±1．69）、（10．45±4．16）μg／L]低于NI组[（23．68±3．85）μg／L，P均〈0．05]，H11组、H12组[（502．67±97．03）、（822．15±139．45）μs／L]均高于NI组（P均〈0．01）。NI组血清碘随孕期进展逐渐降低，但差异无统计学意义（P均〉0．05）。LI1组孕中期、孕晚期羊水碘中位数（0．85、3．00μg／L）均低于同期NI组（3．56、7．91μg／L，P均〈0．01）；L12组孕中、晚期羊水碘中位数与同期NI组比较差异无统计学意义（P均〉0．05）。H11组孕中期、晚期羊水碘中位数（49．59、171．21Iμ/L）均高于同期NI组（P均〈0．01），H12组孕中期、晚期羊水碘中位数（98．76、544．77μg／L）均高于同期NI组（P均〈0．01）。各组大鼠孕晚期羊水碘均高于孕中期（P均〈0．01）。LI1组、U2组

英文摘要：

Objective To study the effects of different iodine intakes on rat iodine metabolism during pregnancy. Methods One hundred and fifty female Wistar rats （body weight 80 - 100 g） were randomly divided into five groups： control group（NI）, lower iodine 1 and 2 groups（LI1 and LI2）, High iodine 1 and 2 groups（HI1 and HI2） by weight, 30 rats in each group. These rats were given deionized water containing different concentrations of iodine, 50（NI）, 0 （LI1）, 5 （LI2）, 3000（HI1） and 10 000 μg/L（HI2）, respectively. After 12 weeks, urine samples were collected before copulation. The rats were sacrificed at the first（6 - 7 days）, second （12 - 13 days） and third trimesters （19 - 20 days）, respectively, serum and amniotic fluid samples were collected. Urinary iodine and iodine level in the fetal amniotic fluid were measured by As^3±-Ce^4± catalytic spectrophotometry. Serum iodine was measured by mild acid digestion method. Results The baseline medians of urinary iodine of LI1 and LI2 groups（5.96, 15.92 μg/L） were significantly lower than that of the NI group（43.75 μg/L, all P 〈 0.01 ）, and the values of HI and HI2 groups（5263.96, 20 389.64 μg/L） were significantly higher than that of the NI group （all P 〈 0.01 ）. The median of urinary iodine during pregnancy was significantly lower than that of the baseline of no pregnancy（all P 〈 0.01 ）. The medians of urinary iodine of the NI group at the first and the second trimesters （28.97,34.34 μg/L） were significantly lower than that of the third trimester（42.31 μg/L, all P 〈 0.01 ）. The means of serum iodine of LI1 and LI2 groups[ （3.68 ± 1.69）, （ 10.45 ± 4.16） μg/L] were significantly lower than that of the NI group[ （23.68 ± 3.85）μg/L, all P 〈 0.05], and the means of serum iodine of HI1 and HT2 groups [ （502.67 ± 97.03）, （822.15 ± 139.45）μg/L] were significantly higher than that of the NI group（all P 〈 0.01）. Although the mean of serum iodine of HI group gra