中文摘要：

目的观察碘对哺乳期大鼠甲状腺和乳腺钠碘转运体（NIS）、胰岛素样生长因子I（IGF—I）、转化生长因子β（TGF-β）mRNA表达的影响，探讨NIS、IGF—I、TGF-βmRNA在哺乳期大鼠甲状腺和乳腺摄碘中的作用。方法选择Wistar大鼠101只，其中雌性80只，雄性21只，体质量80～100g。按体质量将雌性大鼠随机分为5组：对照组（正常饲料、饮含碘50ug／L去离子水），低碘1组、2组（低碘饲料，分别饮用去离子水、含碘5ug／L去离子水），高碘1组、2组（正常饲料，分别饮用含碘3000、10000ug／L去离子水），每组16只。在喂养3个月后，将雌鼠与雄鼠按3：1合笼交配，在产后第5、10天，分别处死各组雌鼠，取甲状腺和乳腺，采用实时荧光定量PCR检测哺乳期大鼠甲状腺和乳腺NIS、IGF—I、TGF-βmRNA表达。结果产后第5天．哺乳期大鼠甲状腺和乳腺的NIS、IGF—I、TGF-βmRNA表达组间比较差异均有统计学意义（NIS：F值分别为631．46、64．91，P均〈0．01；IGF—I：F值分别为11．45、6．56，P均〈0．01；TGF-β：F值分别为291．83、304．53，P均〈0．01）。与对照组大鼠甲状腺和乳腺NIS、IGF—I、TGF—βmRNA[NIS：0．0066±0．0023、（0．1481±0．0711）×10 -2：IGF-I：0．0419±0．0062、0．0542±0．0044；TGF：0．1416±0．0277、0．1670±0．0499]比较，低碘1组[NIS：0．0447±0．0110、（0．3030±0．1831）×10 -1；IGF—I：0．0662±0．0078、0．0902±0．008；TGF-β：0．5514±0．0508、0．6942±0．0367]、2组[NIS：0．0317±0．0081、（0．3017±0．1601）×100；IGF—I：0．0645±0．0054、0．0894±0．0093；TGF-β：0．5292±0．0332、0．6704±0．0277]表达明显升高（P均〈0．01）；高碘1组NISmRNA[0．0043±0．0011、（0．1233±0．0954）X10-2]、2组NISmRNA[0．0037±0．0017、（0．1058±0．0854）×10 -2]表达降低（P均〈0．05），但IGF—I[0．0521±0．0910、0．0715±0．0026；0．0516±0

英文摘要：

Objective To observe the influence of iodine on mRNA expression of iodide transporter （NIS）, insulin-like growth factor I （IGF-I ） and transforming growth factor beta（TGF-β） in thyroid and mammary glands of lactating rats and to explore the role of NIS, IGF- I and TGF-β mRNA in iodine uptake in thyroid and mammary glands of lactating rats. Methods One hundred and one Wistar rats（80 female and 21 male）, weighting 8 - 100 g were selected. These female rats were randomly divided into five groups according to their body weight： control group （NI, normal feed, drank deionized water containing iodine 50 ug/L） ; low iodine group 1 and 2 （LI-1, LI-2, low iodine feed, drank deionized water containing iodine 0 and 5 ug/L, respectively）; high iodine group 1 and 2（HI-1, HI-2, normal feed, drank deionized water containing iodine 3000 and 10 000 ug/L, respectively）, 16 rats in each group. After feeding for 3 months, the female and male rates were mated 3 ： 1. The female rats in each group were sacrificed at the fifth and tenth day after postpartum. Thyroid and mammary glands were taken. The mRNA levels of NIS, IGF- I and TGF-β in thyroid and mammary glands of lactating rats were determined by real time quantitative PCR. Results The fifth days after postartum, NIS, IGF- I and TGF-β mRNA expression levels of thyroid and lactating mammary glands were different between groups, and the differences were statistically significant （NIS： F = 631.46,64.91, all P 〈 0.01; IGF- I ： F = 11.45, 6.56, all P 〈 0.01; TGF-β： F = 291.83, 304.53, all P 〈 0.01）. Compared with control group [NIS： 0.0066 ± 0.0023, （0.1481 ± 0.0711） × 10-2; IGF- I ： 0.0419 ±0.0062, 0.0542 ± 0.0044; TGF-β：0.1416 ± 0.0277, 0.1670 ± 0.0499], regardless of thyroid or mammary gland, the NIS, IGF- I and TGF-β mRNA expression of LI-1[NIS： 0.0447 ± 0.0110, （0.3030 ± 0.1831） × 10-2; IGF-I ： 0.0662 ± 0.0078, 0.0902± 0.008; IGF-I ： 0.5514 ± 0.0508, 0.6942 ± 0.0367], LI-2[NIS： 0.0317 ± 0.0