为研究蚧虫血淋巴中血细胞的种类及其结构特征,采用相差显微镜、荧光显微镜和扫描电镜与透射电镜技术,观察了草履蚧雌成虫血细胞的显微形态与超微结构特征.结果显示,在荧光显微镜和扫描电镜下观察识别出草履蚧血淋巴中的5种血细胞,即:原血胞、浆血胞、粒血胞、囊血胞和类绛色血细胞.在透射电镜下可见,原血胞的细胞核明显,表现出高的细胞质密度;浆血胞最典型的特征是细胞质中有大量的囊泡;粒血胞的细胞质中有发达的粗面内质网和许多玫瑰形的细颗粒.类绛色血细胞最典型的特征是有许多结晶,并在细胞质的空泡区内分布的更多.囊血胞透明性强,具有围核空间,膨大成潴泡状.
For studying the types and structures of blood cells in the haemolymph of scale insects, the morphology and uhrastructure of hemocytes collected form the adult Drosicha corpulenta, Drosicha corpulenta ( Hemiptera : Coccoidea : Monophlebidae) were observed by phase contrast microscope, fluorescence microscope, scanning electron microscopy and transmission electron microscopy. The result showed that five kinds of hemocytes such as prophemocytes, graulocytes, plasmatocytes, cystocytes and oenocytoid were observed in the haemolymph of the scale insect. Their typical characteristics were as follow: prophemoeyte was the smallest one in the five types of the blood cells and it was with a clear cell nucleus and a high dense cytoplasmic ; plasmatocyte was featured with a large number of vesicles in the cytoplasm ; the cytoplasm of graulocytes has developed rough endoplasmic reticulum and many rose-shaped fine particles ; oenocytold was characterized by many crystalline in the cell ; cystocytes has a circumference of nuclear space and swelling into a bubble reterition.