目的研究海洋细菌成团泛菌(Pantoea agglomerans)发酵液的乙酸乙酯萃取物中的化学成分,以期得到有活性的先导化合物。方法采用硅胶柱色谱、凝胶柱色谱、高效液相色谱等方法进行分离纯化,通过理化性质和波谱数据分析鉴定化合物的结构。结果从海洋细菌Pantoea agglomer-ans的乙酸乙酯萃取物中分离得到10个环二肽类化合物,分别鉴定为环-(苯丙氨酸-脯氨酸)[cy-clo(Phe-Pro),1]、环-(缬氨酸-脯氨酸)[cyclo(Val-Pro),2]、环-(丙氨酸-亮氨酸)[cyclo(Ala-Leu),3]、环-(色氨酸-脯氨酸)[cyclo(Trp-Pro),4]、环-(色氨酸-甘氨酸)[cyclo(Trp-Gly),5]、环(4-羟基-脯氨酸-苯丙氨酸)[cyclo(4-hydroxyl-Pro-Phe),6]、环-(脯氨酸-酪氨酸)[cyclo(Pro-Tyr),7]、环-(亮氨酸-亮氨酸)[cyclo(Leu-Leu),8]、环-(脯氨酸-亮氨酸)[cyclo(Pro-Leu),9]、环-(缬氨酸-异亮氨酸)[cyclo(Val-Ile),10]。结论化合物1~10均为首次从海洋细菌Pantoea agglomerans中分离得到。
Objective To isolate and identify the chemical constituents from marine bacterium Pantoea agglomerans.Methods The compounds from the ethyl acetate extracts were isolated by silica gel column,Sephadex LH-20 column chromatography and HPLC techniques.And these compounds were identified by the physico-chemical properties and spectral analysis.Results Ten compounds were isolated from the ethyl acetate extracts of marine bacteria Pantoea agglomerans.They were identified as cyclo(Phe-Pro)(1),cyclo(Val-Pro)(2),cyclo(Ala-Leu)(3),cyclo(Trp-Pro)(4),cyclo(Trp-Gly)(5),cyclo(4-hydroxyl-Pro-Phe)(6),cyclo(Pro-Tyr)(7),cyclo(Leu-Leu)(8),cyclo(Pro-Leu)(9),cyclo(Val-Ile)(10).Conclusions All of the compounds are isolated from the marine bacterium Pantoea agglomerans for the first time.